Human antigen R promotes angiogenesis of endothelial cells cultured with adipose stem cells derived exosomes via overexpression of vascular endothelial growth factor in vitro.

IF 3.5 4区生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2021-12-01 DOI:10.1080/21623945.2021.1982577

Guo Li, Youbai Chen, Yudi Han, Tian Ma, Yan Han

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引用次数: 6

Abstract

Recent studies showed that exosomes obtained from adipose-derived stem cells (ADSCs) could improve the angiogenesis of fat grafts via overexpression of vascular endothelial growth factor (VEGF). Human antigen R (HuR) promotes the expression of VEGF in many cancers, but the effect of HuR in normal endothelial cells in the presence of ADSC-derived exosomes remains unclear. We aimed to investigate the effect of HuR on the expression of VEGF and angiogenesis of human umbilical vein endothelial cells (HUVECs) cultured with ADSCs-derived exosomes. The HuR-overexpressed HUVECs (HuR-HUVECs) were cocultured with ADSCs-derived exosomes. qRT-PCR and Western blotting were performed to examine the stability and expression of VEGF-A mRNA and protein. The proliferation, migration, and proangiogenic capacity of HuR-HUVECs were evaluated using cell counting kit-8 (CCK-8), scratch wound healing, and Matrigel tube formation assay. qRT-PCR showed that HuR-HUVECs had higher expression and slower attenuation of VEGF-A mRNA. Western blotting confirmed higher expression of VEGF-A in HuR-HUVECs. CCK-8, scratch wound healing, and Matrigel tube formation assay demonstrated an increased proangiogenic effect in HuR-HUVECs. HuR promotes angiogenesis of HUVECs cocultured with ADSCs-derived exosomes via stabilization and overexpression of VEGF in vitro. The HuR/VEGF pathway is an important regulatory mechanism of angiogenesis in endothelial cells.

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人抗原R通过体外过表达血管内皮生长因子促进脂肪干细胞来源外泌体培养的内皮细胞的血管生成。

最近的研究表明，从脂肪源性干细胞(ADSCs)中获得的外泌体可以通过过度表达血管内皮生长因子(VEGF)来促进脂肪移植物的血管生成。人抗原R (HuR)在许多癌症中促进VEGF的表达，但在adsc衍生外泌体存在的情况下，HuR在正常内皮细胞中的作用尚不清楚。我们的目的是研究HuR对adscs来源的外泌体培养的人脐静脉内皮细胞(HUVECs) VEGF表达和血管生成的影响。将hr过表达的HUVECs (hr -HUVECs)与adscs衍生的外泌体共培养。采用qRT-PCR和Western blotting检测VEGF-A mRNA和蛋白的表达及稳定性。采用细胞计数试剂盒-8 (CCK-8)、划伤愈合和Matrigel管形成实验评估hr - huvecs的增殖、迁移和促血管生成能力。qRT-PCR结果显示，hr - huvecs具有较高的VEGF-A mRNA表达和较慢的衰减。Western blotting证实VEGF-A在hr - huvec中表达较高。CCK-8、划伤愈合和Matrigel管形成试验表明，HuR-HUVECs的促血管生成作用增强。在体外，HuR通过稳定和过表达VEGF促进与adscs来源的外泌体共培养的HUVECs血管生成。HuR/VEGF通路是内皮细胞血管生成的重要调控机制。

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来源期刊

Adipocyte Medicine-Histology

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

32 weeks

期刊介绍： Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.