Local inflammatory mediators alterations induced by Daboia siamensis venom

IF 3.6 Q2 TOXICOLOGY Toxicon: X Pub Date : 2021-11-01 DOI:10.1016/j.toxcx.2021.100085
Suchitra Khunsap , Kanyanat Promruangreang , Sunutcha Suntrarachun , Jureeporn Noiphrom , Orawan Khow
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Abstract

The ability of Russell's viper (Daboia siamensis) venom (total RVV) and phospholipase A2 (purified PLA2) to induce the local pathological effects were investigated by the local inflammatory events and the release of inflammatory mediators. Both 0.5 μg of total RVV/mouse and 0.15 μg of purified PLA2/mouse were administered via intra-peritoneal injection. After 30 min, 1 h, 2 h, and 4 h incubation time, the peritoneal cavity was flooded with normal saline and the total leukocytes were collected. The eicosanoids (lipid mediators) and the leukocyte expression of cyclooxygenase (COX-1 and COX-2) were investigated by ELISA assay and western blotting, respectively. The amounts of total leukocytes were increased from 30 min to 2 h, then decreased at 4 h, by both total RVV and purified PLA2. Both treatments also induced the expression of COX-2 which was increased at 2 h and then decreased at 4 h, whereas only purified PLA2 induced the expression level of a COX-1 protein which was increased at 30 min, then constantly expressed until 4 h. In addition, total RVV and purified PLA2 caused the release of the eicosanoids; PGE2, TXB2, and LTB4, which reached the peak after 2 h. The findings of this study indicate that purified PLA2 has the potential effects to induce the local inflammation relating the amounts of leukocytes cells, lipid mediators and COX-2 more than the total RVV.

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大鲵毒液诱导局部炎症介质的改变
通过局部炎症事件和炎症介质的释放,研究了罗素蝰蛇毒液(总RVV)和磷脂酶A2(纯化PLA2)诱导局部病理效应的能力。腹腔注射总RVV 0.5 μg /只,纯化PLA2 0.15 μg /只。孵育30 min、1 h、2 h、4 h后,腹腔灌注生理盐水,收集白细胞总数。采用酶联免疫吸附法(ELISA)和免疫印迹法(western blotting)分别检测各组小鼠脂质介质类二十烷酸和白细胞环氧化酶(COX-1和COX-2)的表达。总RVV和纯化PLA2在30分钟至2小时内使白细胞总数增加,然后在4小时时减少。两种处理均诱导COX-2的表达,在2 h时升高,在4 h时降低,而纯化PLA2只诱导COX-1蛋白的表达,在30 min时升高,并持续表达至4 h。此外,总RVV和纯化PLA2均引起类二十烷酸的释放;PGE2、TXB2和LTB4在2 h后达到峰值。本研究结果表明纯化PLA2具有诱导局部炎症的潜在作用,其白细胞、脂质介质和COX-2的数量大于RVV总量。
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来源期刊
Toxicon: X
Toxicon: X Pharmacology, Toxicology and Pharmaceutics-Toxicology
CiteScore
6.50
自引率
0.00%
发文量
33
审稿时长
14 weeks
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