KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media.

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Poultry Science Pub Date : 2021-10-25 DOI:10.2141/jpsa.0200085
Masafumi Katayama, Manabu Onuma, Tomokazu Fukuda
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引用次数: 1

Abstract

Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.

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kw -1比DMEM和199培养基更适合于鸡成纤维细胞培养。
培养的细胞对家禽科学家来说是一个有用的资源,因为这些细胞使科学家能够在体外评估对传染病等条件的生物反应,同时模拟鸟类的全身反应。然而,禽类细胞培养需要一种优化的基础培养基,目前这种基础培养基的选择相对较少(培养基199和kw -1)。这意味着禽细胞培养的最佳基础培养基仍有发展的空间。在这里,我们比较了kw -1培养基、Dulbecco's modified Eagle培养基(DMEM)和199培养基在鸡成纤维细胞培养过程中的作用,并确定kw -1培养基仍然是这些试验的最佳培养基。我们的研究结果表明,与DMEM和medium 199相比,在kw -1培养基中培养的成纤维细胞的DNA损伤减少,并且与DMEM和medium 199相比,这些细胞在kw -1培养基中也表现出更好的生长动力学。据我们所知,这是第一个描述禽类细胞培养基比较分析的研究,这将为家禽科学家提供有用的信息。
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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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