Environmental risk assessment of the DvSSJ1 dsRNA and the IPD072Aa protein to non-target organisms.

IF 4.5 2区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2021-01-02 Epub Date: 2021-12-14 DOI:10.1080/21645698.2021.1982348
Chad J Boeckman, Jennifer A Anderson, Christopher Linderblood, Taylor Olson, Jason Roper, Kristine Sturtz, Carl Walker, Rachel Woods
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Abstract

Event DP-Ø23211-2 (hereafter referred to as DP23211) maize expresses the DvSSJ1 double-stranded RNA (DvSSJ1 dsRNA) and the IPD072Aa protein, encoded by the ipd072Aa gene. DvSSJ1 dsRNA and the IPD072Aa protein each provide control of corn rootworms (Diabrotica spp.) when expressed in plants. As part of the environmental risk assessment (ERA), the potential hazard to non-target organisms (NTOs) exposed to the DvSSJ1 dsRNA and the IPD072Aa protein expressed in DP23211 maize was assessed. Worst-case estimated environmental concentrations (EECs) for different NTO functional groups (pollinators and pollen feeders, soil dwelling detritivores, predators and parasitoids, aquatic detritivores, insectivorous birds, and wild mammals) were calculated using worst-case assumptions. Several factors that reduce exposure to NTOs under more realistic environmental conditions were applied, when needed to provide more environmentally relevant EECs. Laboratory bioassays were conducted to assess the activity of DvSSJ1 dsRNA or the IPD072Aa protein against selected surrogate species, and margins of exposure (MOEs) were calculated by comparing the Tier I hazard study results to worst-case or refined EECs. Based on specificity and MOE values, DvSSJ1 dsRNA and the IPD072Aa protein expressed in DP23211 maize are not expected to be harmful to NTO populations at environmentally relevant concentrations.

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DvSSJ1 dsRNA 和 IPD072Aa 蛋白对非目标生物的环境风险评估。
事件 DP-Ø23211-2(以下简称 DP23211)玉米表达了 DvSSJ1 双链 RNA(DvSSJ1 dsRNA)和 IPD072Aa 基因编码的 IPD072Aa 蛋白。DvSSJ1 dsRNA 和 IPD072Aa 蛋白在植物中表达时,可分别控制玉米根虫(Diabrotica spp.)。作为环境风险评估 (ERA) 的一部分,评估了暴露于在 DP23211 玉米中表达的 DvSSJ1 dsRNA 和 IPD072Aa 蛋白的非目标生物 (NTO) 的潜在危害。利用最坏情况假设,计算了不同 NTO 功能群(传粉者和花粉取食者、土壤中的食肉动物、捕食者和寄生虫、水生食肉动物、食虫鸟类和野生哺乳动物)的最坏情况估计环境浓度 (EEC)。必要时,还应用了一些可减少在更现实的环境条件下接触 NTO 的因素,以提供与环境更相关的环境经济系数。进行了实验室生物测定,以评估 DvSSJ1 dsRNA 或 IPD072Aa 蛋白质对选定替代物种的活性,并通过比较 I 级危害研究结果与最坏情况或改进后的 EECs 计算暴露限值 (MOE)。根据特异性和暴露限值,在 DP23211 玉米中表达的 DvSSJ1 dsRNA 和 IPD072Aa 蛋白在环境相关浓度下不会对 NTO 群体造成危害。
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来源期刊
Gm Crops & Food-Biotechnology in Agriculture and the Food Chain
Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
8.10
自引率
10.30%
发文量
22
期刊介绍: GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments
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