Trehalose glycopolymers for cryopreservation of tissue-engineered constructs

IF 2.3 3区 生物学 Q2 BIOLOGY Cryobiology Pub Date : 2022-02-01 DOI:10.1016/j.cryobiol.2021.11.004
Jin Wang, Xiaodi Shi, Minghao Xiong, Wen-Song Tan, Haibo Cai
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引用次数: 5

Abstract

The development of an effective cryopreservation method to achieve off-the-shelf and bioactive tissue-engineered constructs (TECs) is important to meet the requirements for clinical applications. The trehalose, a non-permeable cryoprotectant (CPA), has difficulty in penetrating the plasma membranes of mammalian cells and has only been used in combination with other cell penetrating CPA (such as DMSO) to cryopreserve mammalian cells. However, the inherent cytotoxicity of DMSO results in increasing risks with respect to cryopreserved cells. Therefore, in this study, permeable trehalose glycopolymers were synthesised for cryopreservation of TECs. The trehalose glycopolymers exhibited good ice inhibiting activities and biocompatibilities. Furthermore, the viability and function of TECs after cryopreservation with 5.0 wt% S2 were similar to those of the non-cryopreserved TECs. We developed an effective preservation strategy for the off-the-shelf availability of TECs.

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用于组织工程构建物低温保存的海藻糖共聚物
开发一种有效的冷冻保存方法来获得现成的生物活性组织工程构建物(TECs)对于满足临床应用的要求非常重要。海藻糖是一种非渗透性冷冻保护剂(CPA),难以穿透哺乳动物细胞的质膜,并且仅与其他细胞穿透性冷冻保护剂(如DMSO)联合使用以低温保存哺乳动物细胞。然而,DMSO固有的细胞毒性导致冷冻保存细胞的风险增加。因此,本研究合成了可渗透海藻糖共聚物,用于TECs的低温保存。海藻糖共聚物具有良好的抑冰活性和生物相容性。此外,5.0 wt% S2冷冻保存后的TECs的活力和功能与未冷冻保存的TECs相似。我们开发了一种有效的保存策略,以确保TECs的现货可用性。
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来源期刊
Cryobiology
Cryobiology 生物-生理学
CiteScore
5.40
自引率
7.40%
发文量
71
审稿时长
56 days
期刊介绍: Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.
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