Cryobiology最新文献

{"title":"In Memoriam: Dr. Arthur W. Rowe.","authors":"William A Rowe, Chris Rowe Taitt","doi":"10.1016/j.cryobiol.2026.105587","DOIUrl":"https://doi.org/10.1016/j.cryobiol.2026.105587","url":null,"abstract":"","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":" ","pages":"105587"},"PeriodicalIF":2.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of artificial intelligence in cryopreservation: Technological advances and future challenges.","authors":"Xingyue Lei, Liqun He, Gang Zhao","doi":"10.1016/j.cryobiol.2026.105589","DOIUrl":"https://doi.org/10.1016/j.cryobiol.2026.105589","url":null,"abstract":"<p><p>This review systematically sorts out the latest research progress and application status of artificial intelligence (AI) technology in the field of cryopreservation, with a focus on its action mechanisms in aspects such as protocol optimization, damage prediction, and quality control in the cryopreservation of biological samples like cells, embryos, and tissues. From the perspectives of cross-scale modeling, dynamic process optimization, and prediction of structural and functional states, the article analyzes the advantages and challenges of AI in improving the efficiency of cryopreservation, reducing cell damage, and increasing the survival rate after resuscitation. It also summarizes the key application cases in the design of cryoprotectant (CPA) formulations, the control of the freezing or thawing process, and the monitoring of long-term stability. In view of the deficiencies of current AI models in sample diversity, mechanism analysis, and clinical translation, the review puts forward future development directions, including multi-modal data fusion, enhanced interpretability, and the construction of intelligent adaptive control systems. This research provides a reference for the intelligent development of cryopreservation technology and lays a theoretical foundation for subsequent clinical and industrial applications.</p>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"105589"},"PeriodicalIF":2.1,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146124265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective cryopreservation of western capercaillie (Tetrao urogallus) semen using dimethylsulphoxide.","authors":"Alberto Gómez-Crespo, Artur Kowalczyk, Julián Santiago-Moreno, Ewa Łukaszewicz","doi":"10.1016/j.cryobiol.2026.105590","DOIUrl":"https://doi.org/10.1016/j.cryobiol.2026.105590","url":null,"abstract":"<p><p>This work examines the use of Me₂SO as a cryoprotective agent (CPA) for capercaillie semen, comparing it with the use of dimethyformamide (DMF). Thirty semen samples were collected from seven males, diluted and divided into two aliquots before adding either Me₂SO (final concentration 8 %) or DMF (6 %). These samples were then frozen in liquid nitrogen vapour. No significant differences were seen between the Me₂SO and DMF treatments with respect to any frozen-thawed sperm motility characteristics, and plasma membrane integrity, although the proportion of viable spermatozoa showing normal morphology was greater (P < 0.05) with the DMF treatment. The proportion of sperm showing a bent neck was greater in samples frozen with Me₂SO than in fresh sperm (P < 0.001), and than in samples frozen with DMF (P < 0.05). Thus, although Me₂SO appears to be a suitable CPA for freezing capercaillie semen, some variables are slightly better when DMF is used.</p>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"105590"},"PeriodicalIF":2.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of cryopreservation on pollen viability and morphological integrity of five Indian Crinum species","authors":"Harshid Pulparambil ,&nbsp;P.E. Rajasekharan ,&nbsp;N.S. Pradeep","doi":"10.1016/j.cryobiol.2026.105586","DOIUrl":"10.1016/j.cryobiol.2026.105586","url":null,"abstract":"<div><div><em>Crinum</em> species are ornamental plants of high horticultural value and medicinal importance, known for their bioactive alkaloids with neurological and anticancer properties. Sustainable use and genetic conservation of <em>Crinum</em> require reliable long-term germplasm conservation strategies, and pollen cryopreservation offers an effective approach by enabling year-round breeding and conservation independent of seasonal and geographic constraints. This study presents the first species-comparative evaluation of pollen germination behaviour and cryopreservation responses in five ecologically contrasting Indian <em>Crinum</em> species (<em>C. asiaticum</em>, <em>C. latifolium, C. malabaricum</em>, <em>C. lorifolium and C. viviparum</em>). <em>In vitro</em> pollen germination treatments with varying sucrose (1–10 %) and polyethylene glycol (PEG) concentrations were optimized, species-specific critical moisture contents were determined, and pollen viability was assessed before and after cryostorage at −196 °C for up to 180 days. Distinct, habitat-linked germination responses were observed: aquatic and semi-aquatic species achieved optimal germination (&gt;91 %) only under PEG-mediated osmotic regulation, whereas terrestrial species performed best in sucrose-only treatments. Precise moisture content ranges (12–18 %) were identified as critical for maximizing post-cryopreservation viability, with small deviations resulting in marked germination loss. Cryopreserved pollen retained high functional competence, with minimal viability decline (&lt;5 %) after 180 days, and scanning electron microscopy revealed only minor, non-critical ultrastructural changes. By linking pollen osmotic sensitivity, ecological adaptation, and cryotolerance within a single genus, this study advances pollen cryobiology beyond empirical optimization and provides biologically informed, species-specific protocols for pollen cryobanking. These findings strengthen the scientific basis for <em>ex situ</em> conservation, germplasm exchange, and breeding programmes targeting horticultural and medicinal improvement of <em>Crinum</em> species.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105586"},"PeriodicalIF":2.1,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An effective method for recovering rat spermatogonial stem cells from frozen rat testis cells","authors":"Fan Yang ,&nbsp;Yaru Li ,&nbsp;Mary R. Avarbock ,&nbsp;Eoin C. Whelan ,&nbsp;Ralph L. Brinster","doi":"10.1016/j.cryobiol.2025.105581","DOIUrl":"10.1016/j.cryobiol.2025.105581","url":null,"abstract":"<div><div>Cryopreservation of testis tissue, including spermatogonial stem cells (SSCs), underpins a potential method of fertility preservation for prepubertal males undergoing gonadotoxic treatments. A key understudied factor in the recovery of high viability testis cell populations from frozen is the method of thawing and purification of SSCs. Here, we thawed rat testis cells, testing the effect of gradual dilution with DMSO, supplementation with sugars and removal of dead cells on thawing efficiency. Gradient reduction of DMSO and trehalose supplementation increased thawing viability significantly. Magnetic-activated cell sorting of EpCAM⁺ cells enriched rat SSCs with a concentration superior to that achieved with laminin selection. A combination of methods to remove dead cells and enrich rat SSCs from post thaw rat testis cells provided an optimized protocol. Taken together, we have established a reliable protocol to enrich high-viability SSCs from post thaw rat testis cells, which may serve as a template for purifying SSCs from frozen human testis cells.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105581"},"PeriodicalIF":2.1,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The histologic and molecular alterations during the cold storage of the ascending human colon","authors":"Jasmine Bagge ,&nbsp;Gustav Hagberg ,&nbsp;Anna Ermund ,&nbsp;Anna Casselbrant ,&nbsp;Edina Sehiç ,&nbsp;John Mackay Søfteland ,&nbsp;Mats Hellström ,&nbsp;Gustaf Herlenius ,&nbsp;Mihai Oltean","doi":"10.1016/j.cryobiol.2026.105585","DOIUrl":"10.1016/j.cryobiol.2026.105585","url":null,"abstract":"<div><div>Inclusion of a colon segment in visceral grafts is increasing as this may improve water and electrolyte absorption and ultimately improve recipients’ renal function. In contrast with the vast knowledge on the ischemia/reperfusion injury in the small bowel, similar data on the colon is almost inexistent. Using light microscopy, immunofluorescence and Western blot, we assessed several histological and molecular changes following the cold storage (CS) of the ascending colon of 18 human brain-dead organ donors for up to 24 h. CS induced progressive yet limited epithelial detachment and goblet cell depletion. Tight junction proteins claudin-1, claudin-3, claudin-4 and occludin were well preserved during the first 14 h of cold storage but its tissue expression decreased following 24 h of CS as evidenced by Western blot and immunofluorescence. ZO-1 expression remained unchanged throughout the 24 h of CS and showed a strong and continuous immunostaining along the entire epithelial lining. Expression of Na+/H+ exchanger (NHE)-1 and 2 remained unaffected by CS. Enterocyte apoptosis increased significantly after 14 h of CS. The current data indicate that colonic mucosa can withstand at least 14 h of cold ischemia without significant histological or molecular changes and implies that the small bowel remains the most vulnerable part of a prospective visceral allograft.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105585"},"PeriodicalIF":2.1,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized methods for the functional cryopreservation of adult human primary cardiomyocytes","authors":"Zhimin Wang ,&nbsp;Chao Tong ,&nbsp;Miaomiao Xu ,&nbsp;Shanshan Feng ,&nbsp;Mengqi Dong ,&nbsp;Rongjia Rao ,&nbsp;Xianqiang Wang ,&nbsp;Wei Feng ,&nbsp;Changwei Zhang ,&nbsp;Zhan Hu ,&nbsp;Li Wang ,&nbsp;Shengshou Hu ,&nbsp;Bingying Zhou","doi":"10.1016/j.cryobiol.2026.105583","DOIUrl":"10.1016/j.cryobiol.2026.105583","url":null,"abstract":"<div><div>Adult human primary cardiomyocytes (hPCMs) is a high-fidelity and informative cardiac model that is expected to advance our knowledge of the human heart. However, currently, no method exists that recovers hPCMs from cryopreservation with high efficiency, limiting their use as a versatile research model. Based on our previous success with isolated hPCMs, we designed a new strategy that cryopreserves myocardial tissue at a specific step during hPCM dissociation, i.e., after sectioning tissue chunks into tissue slices. This method yielded cell viabilities comparable to that of freshly isolated hPCMs, and surpassed the performance of cell isolation from cryopreserved tissue chunk, as well as recovery of frozen isolated cells. We demonstrate cytoskeletal, ultrastructural, transcriptomic, metabolic, electrophysiological recovery of these cells both over short-term (1 week) and long-term (6–12 months) storage. In addition, these cells responded promptly to external stimuli, including adrenergic stimulation and hypoxic stress, suggesting functional integrity. Our study illustrates an optimized cryopreservation protocol for the functional recovery of cardiomyocytes from myocardial tissue, broadening their applications in basic and translational research, and opens up the possibility for cell banking.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105583"},"PeriodicalIF":2.1,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of FBS-DMSO cryoprotectant composition in artificial microbiome models mimicking key gut microbiota enterotypes","authors":"L.V. Zalomova,&nbsp;E.E. Fesenko Jr.","doi":"10.1016/j.cryobiol.2026.105582","DOIUrl":"10.1016/j.cryobiol.2026.105582","url":null,"abstract":"<div><div>The ratio of microorganisms in the composition of the microflora of the small and large intestines plays a crucial role in human health. Therefore, it is essential to preserve the original proportions of species over an extended period for their further therapeutic application. It has previously been established that three primary enterotypes dominate the human gut microbiota: <em>Bacteroides</em>, <em>Prevotella</em>, and <em>Ruminococcus</em>. However, there is no precise information on how their species ratios are affected by deep freezing. In our study, we examined the preservation of the ratios of microorganisms in the human gut before and after cryopreservation, represented as distinct clusters consisting of four different bacterial species dominant in the gut microbiome. Using photometric registration of optical density and fluorescent staining methods, we demonstrated that the viability of most bacteria remained high in the cryoprotective medium of 5 % Me₂SO/FBS. Additionally, the calculation of the Pattern Comparison Index (PCI) showed good results in maintaining the community structure of bacteria in each of the artificial models. Thus, this modeling of microbiocenoses allows for the identification of patterns in the preservation of their quantitative composition during long-term storage in liquid nitrogen.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105582"},"PeriodicalIF":2.1,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The post-thaw quality, antioxidant activity, and in vivo fertility of Zaraibi buck semen frozen-stored in the presence of different concentrations of either quercetin or L-arginine","authors":"Essam A. Almadaly ,&nbsp;Mohamed A. Elbendary ,&nbsp;Samia M. Abd El-Rheem ,&nbsp;Ahmed M. Shehabeldin ,&nbsp;Wael B. El-Domany ,&nbsp;Adel A. Ramoun","doi":"10.1016/j.cryobiol.2025.105580","DOIUrl":"10.1016/j.cryobiol.2025.105580","url":null,"abstract":"<div><div>Over the last decade, there has been a growing interest in incorporating specific additives into buck semen extenders to enhance their semen quality, as their antioxidant defense against oxidative stress and lipid peroxidation is insufficient. Due to their strong antioxidant capabilities, quercetin (QUE) and L-arginine (LA) have garnered considerable interest among these additives. Therefore, this study investigates the effect of adding various concentrations of either QUE or LA in the cryopreservation extender on the post-thaw sperm characteristics, kinematics, enzymatic antioxidant activity, and <em>in vivo</em> fertility of buck semen. Ejaculates were collected from 9 healthy <em>Zaraibi</em> bucks using an electroejaculator once a week. Good-quality ejaculates were pooled and dispensed into 7 aliquots; each aliquot was diluted with Tris-egg yolk citrate extender containing: 1) 10 μM QUE; 2) 15 μM QUE; 3) 30 μM QUE; 4) 50 μM QUE; 5) 2 mM LA; 6) 4 mM LA; and 7) the last aliquot was not supplemented with any additive and set as a control (CTRL). Diluted semen samples were equilibrated at 4 °C for 4 h, loaded into French mini straws, sealed, and frozen-stored in liquid nitrogen. Frozen straws were thawed and examined for sperm characteristics and kinematics; also, enzymatic antioxidants, including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC), as well as the lipid peroxidation marker malondialdehyde (MDA), were determined. A total of 210 (30 female/group) mature and healthy doe-goats were selected, exposed to estrus synchronization, and inseminated by the prepared frozen-thawed straws to calculate their <em>in vivo</em> fertility rates. The obtained findings revealed that the 30 μM QUE, 50 μM QUE, and 4 mM LA groups had the highest proportions (P &lt; 0.05) of all post-thaw sperm characteristics. Only the 30 μM QUE group had the greatest (P &lt; 0.05) values of all sperm kinematics. Frozen-thawed buck semen's enzymatic antioxidant activity was markedly enhanced by adding either 30 μM QUE or 4 mM LA into the semen extender. The <em>in vivo</em> fertility rates of frozen-thawed straws enriched with either 30 μM QUE (73.33 %) or 4 mM LA (70.00 %) were higher than those of other treatments and the control group (43.33 %). In conclusion, adding either 30 μM QUE or 4 mM LA to the buck semen cryopreservation extender is recommended to improve its post-thaw sperm quality, antioxidant activity, and <em>in vivo</em> fertility.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105580"},"PeriodicalIF":2.1,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145910919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adding lycopene to the freezing media enhances the quality, antioxidant capacity, and fertilization ability of frozen-thawed Oure-type Tibetan sheep (Ovis aries) sperm","authors":"Yujie Tang ,&nbsp;Hong Wu ,&nbsp;Lidan Liu ,&nbsp;Yanbing Liu ,&nbsp;Haoqiang Qin ,&nbsp;Mingxia Li ,&nbsp;Deqing Yan ,&nbsp;Chengtu Zhang ,&nbsp;Jianmin Su","doi":"10.1016/j.cryobiol.2025.105578","DOIUrl":"10.1016/j.cryobiol.2025.105578","url":null,"abstract":"<div><div>Lycopene (LYC) is a plant-derived antioxidant that ameliorates oxidative and other stress-related damage in spermatozoa yet its inclusion in ovine freezing medium remains largely unexplored. In this study semen was collected from eight Oura-type Tibetan sheep with three ejaculates per male and cryopreserved in freezing medium supplemented with 0, 1, 2, 4, 8 and 16 μM LYC to identify the optimal concentration and evaluate its effects on structural integrity antioxidant status and fertilizing capacity. A dose of 2 μM LYC proved optimal markedly improving post-thaw survival and motion kinetics relative to the control (<em>P</em> &lt; 0.01). At both 25 °C and 4 °C this concentration prolonged survival and increased the proportion of live cells at each observation point. It also enhanced acrosome (<em>P</em> &lt; 0.01), head membrane (<em>P</em> &lt; 0.05) and tail membrane (<em>P</em> &lt; 0.01) integrity and elevated mitochondrial membrane potential (MMP) (<em>P</em> &lt; 0.05). Compared with the control the 2 μM LYC group showed lower reactive oxygen species (ROS) and malondialdehyde (MDA) levels (<em>P</em> &lt; 0.01) and higher superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) (<em>P</em> &lt; 0.05 and <em>P</em> &lt; 0.01 respectively). <em>In vitro</em> fertilization (IVF) trials revealed that 2 μM LYC increased cleavage (<em>P</em> &lt; 0.05) and blastocyst formation rates (<em>P</em> &lt; 0.01) without affecting blastocyst cell number (<em>P</em> &gt; 0.05). In a cervical artificial insemination (AI) programme pregnancy rates were 44 % for the 2 μM LYC group versus 28 % for the control. We conclude that adding 2 μM LYC to the freezing medium improves post-thaw quality counters oxidative stress and enhances the fertilizing capacity of Oura-type Tibetan sheep spermatozoa.</div></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"122 ","pages":"Article 105578"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}