-808 nm-activated Ca²⁺doped up-conversion nanoparticles that release no inducing liver cancer cell (HepG2) apoptosis.

IF 2.4 3区化学 Q3 CHEMISTRY, ANALYTICAL Methods and Applications in Fluorescence Pub Date : 2022-02-23 DOI:10.1088/2050-6120/ac5524

Xinmeng Fa, Shaowei Lin, Jianghua Yang, Chong Shen, Yuanli Liu, Yongyang Gong, Aimiao Qin, Jun Ou, Ute Resch-Genger

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引用次数: 3

Abstract

A near-infrared (NIR) light-triggered release method for nitric oxide (NO) was developed utilizing core/shell NaYF₄: Tm/Yb/Ca@NaGdF₄: Nd/Yb up-conversion nanoparticles (UCNPs) bearing a mesoporous silica (mSiO₂) shell loaded with the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). To avoid overheating in biological samples, Nd³⁺was chosen as a sensitizer, Yb³⁺ions as the bridging sensitizer, and Tm³⁺ions as UV-emissive activator while co-doping with Ca²⁺was done to enhance the luminescence of the activator Tm³⁺. NO release from SNAP was triggered by an NIR-UV up-conversion process, initiated by 808 nm light absorbed by the Nd³⁺ions. NO release was confirmed by the Griess method. Under 808 nm irradiation, the viability of the liver cancer cell line HepG2 significantly decreased with increasing UCNPs@mSiO₂-SNAP concentration. For a UCNPs@mSiO₂-SNAP concentration of 200μg ml^-1, the cell survival probability was 47%. These results demonstrate that UCNPs@mSiO₂-SNAP can induce the release of apoptosis-inducing NO by NIR irradiation.

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-808纳米激活的Ca2+掺杂上转换纳米颗粒释放不诱导肝癌细胞(HepG2)凋亡。

利用核/壳层NaYF4: Tm/Yb/Ca@NaGdF4: Nd/Yb上转化纳米颗粒(UCNPs)，制备了一种近红外(NIR)光触发释放一氧化氮(NO)的方法，该纳米颗粒具有介孔二氧化硅(mSiO2)壳层，负载NO供体s -亚硝基-n -乙酰基- l-青霉菌胺(SNAP)。为了避免生物样品过热，选择Nd3+作为敏化剂，Yb3+离子作为桥接敏化剂，Tm3+离子作为uv发射激活剂，并与Ca2+共掺杂以增强激活剂Tm3+的发光能力。SNAP的NO释放是由NIR-UV上转换过程触发的，该过程是由Nd3+离子吸收808 nm光引发的。Griess法证实NO释放。808 nm照射下，肝癌细胞株HepG2的活力随着UCNPs@mSiO2-SNAP浓度的增加而显著降低。UCNPs@mSiO2-SNAP浓度为200μg ml-1时，细胞存活率为47%。这些结果表明UCNPs@mSiO2-SNAP可以诱导近红外照射释放诱导凋亡的NO。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL

CiteScore

6.20

自引率

3.10%

发文量

期刊介绍： Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.