TRIB3 Promotes Osteogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells Levelled by Post-transcriptional Regulation of miR-24-3p.

Xiang Song Bai, Ping Zhang, Yun Song Liu, Hao Liu, Long Wei Lv, Yong Sheng Zhou
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引用次数: 1

Abstract

Objective: To explore the effect of TRIB3 on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASCs) and reveal the potential role of TRIB3 in bone regeneration.

Methods: TRIB3-knockdown and TRIB3-overexpression hASCs were used to explore the effect of TRIB3 on osteogenic differentiation by alkaline phosphatase (ALP) staining, alizarin red S (ARS) staining, quantitative real-time polymerase chain reaction (qRT-PCR) and heterotopic bone formation. The regulation of miR-24-3p on TRIB3 was detected by qRT-PCR and western blot. Ribonucleic acid (RNA) sequencing was performed to investigate the downstream regulatory network of TRIB3.

Results: TRIB3 promoted the osteogenic differentiation of hASCs both in vitro and in vivo. This process was regulated epigenetically by the post-transcriptional regulation of miR-24-3p, which could bind directly to the three prime untranslated region (3'UTR) of TRIB3 and inhibit TRIB3 expression. The downstream regulatory network of TRIB3-mediated osteogenic differentiation was related to calcium ion binding and cell metabolism, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and nuclear factor-κB (NF-κB) signalling pathways.

Conclusion: TRIB3 is a promising therapeutic target for hASC-based bone tissue engineering and the epigenetic regulation of TRIB3 through miR-24-3p permits regulatory controllability, thus promoting osteogenesis through an important metabolic target while obtaining a safe and controllable effect via post-transcriptional epigenetic regulation.

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TRIB3通过miR-24-3p转录后调控促进人脂肪源性间充质干细胞成骨分化。
目的:探讨TRIB3对人脂肪源性间充质干细胞(hASCs)成骨分化的影响,揭示TRIB3在骨再生中的潜在作用。方法:采用碱性磷酸酶(ALP)染色、茜素红S (ARS)染色、实时定量聚合酶链反应(qRT-PCR)和异位成骨方法,采用TRIB3敲低和TRIB3过表达的hASCs,探讨TRIB3对成骨分化的影响。采用qRT-PCR和western blot检测miR-24-3p对TRIB3的调控作用。核糖核酸(RNA)测序研究了TRIB3的下游调控网络。结果:TRIB3在体外和体内均能促进hASCs的成骨分化。这一过程受miR-24-3p转录后调控的表观遗传调控,miR-24-3p可以直接结合TRIB3的3′非翻译区(3’utr),抑制TRIB3的表达。trib3介导的成骨分化的下游调控网络与钙离子结合和细胞代谢、细胞外信号调节蛋白激酶1和2 (ERK1/2)和核因子-κB (NF-κB)信号通路有关。结论:TRIB3是一个很有前景的基于hasc的骨组织工程治疗靶点,通过miR-24-3p对TRIB3进行表观遗传调控具有调控可控性,从而通过重要的代谢靶点促进成骨,同时通过转录后的表观遗传调控获得安全可控的效果。
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