Automated capillary flow segmentation and mapping for nailfold video capillaroscopy

IF 1.9 4区医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2022-02-25 DOI:10.1111/micc.12753

Tomoya Niizawa, Kota Yokemura, Tomoya Kusaka, Takuma Sugashi, Ichiro Miura, Keiji Kawagoe, Kazuto Masamoto

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引用次数: 2

Abstract

Objective

This study aimed to develop an automated image analysis method for segmentation and mapping of capillary flow dynamics captured using nailfold video capillaroscopy (NVC). Methods were applied to compare capillary flow structures and dynamics between young and middle-aged healthy controls.

Methods

NVC images were obtained in a resting state, and a region of the vessel in the image was extracted using a conventional U-Net neural network. The approximate length, diameter, and radius of the curvature were calculated automatically. Flow speed and its fluctuation over time were mapped using the Radon transform and frequency spectrum analysis from the kymograph image created along the vessel's centerline.

Results

The diameter of the curve segment (14.4 μm and 13.0 μm) and the interval of two straight segments (13.7 μm and 32.1 μm) of young and middle-aged subjects, respectively, were significantly different. Faster flow was observed in older subjects (0.48 mm/s) than in younger subjects (0.26 mm/s). The power spectral analysis revealed a significant correlation between the high-frequency power spectrum and the flow speed.

Conclusions

The present method allows a spatiotemporal characterization of capillary morphology and flow dynamics with NVC, allowing a wide application such as large-scale health assessment.

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自动毛细管流分割和映射的甲襞视频毛细管镜

目的建立一种自动图像分析方法，用于对甲襞视频毛细血管镜(NVC)拍摄的毛细血管流动动态进行分割和映射。方法比较中青年健康对照者的毛细血管流动结构和动力学。方法获取静息状态下的NVC图像，利用传统的U-Net神经网络提取图像中的血管区域。自动计算出曲率的大致长度、直径和半径。通过Radon变换和频谱分析，利用沿船舶中心线生成的测速仪图像绘制了流速及其随时间的波动。结果中青年受试者的曲线段直径(14.4 μm和13.0 μm)和两条直线段间距(13.7 μm和32.1 μm)差异有统计学意义。老年受试者的血流速度(0.48 mm/s)高于年轻受试者(0.26 mm/s)。功率谱分析表明，高频功率谱与流速之间存在显著的相关关系。结论该方法可以利用NVC对毛细血管形态和血流动力学进行时空表征，可广泛应用于大规模健康评估。

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.

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