Ahmed Al Ghaithi, Atika Al Bimani, Sultan Al Maskari
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引用次数: 1
Abstract
Background: Isolation of the causal microorganisms in osteomyelitis presents a major challenge for treating clinicians. Several methods have been proposed to rapidly and accurately identify microorganisms. There has been an increasing interest in using Raman spectroscopy in the field of microbial detection and characterisation. This paper explores the use of Raman spectroscopy identification as one of the most difficult-to-isolate microorganisms causing osteomyelitis.
Methods and results: Fresh healthy human bone samples were collected from patients undergoing a total knee replacement. These samples were then inoculated with fresh overnight Pseudomonas aeruginosa (PAO) cultures. Bacteria growth and bone ultrastructural changes were monitored over a period of 6 weeks. The experiment demonstrated ultrastructural bony destruction caused by osteolytic PAO secretions. Raman-specific spectral signatures related to the cellular membranes of PAO structures were spotted indicating survival of bacteria on the bone surface.
Conclusion: This study showed the promising ability of Raman spectroscopy to identify the presence of bacteria on the surface of inoculated bone samples over time. It was able to detect the osteolytic activity of the bacteria as well as ultrastructure specific to PAO virulence. This method may have a role as an aid to existing diagnostic methods for fast and accurate bacterial identification in bone infection.
How to cite this article: Al Ghaithi A, Al Bimani A, Al Maskari S. Investigating the Growth of Pseudomonas aeruginosa and Its Influence on Osteolysis in Human Bone: An In Vitro Study. Strategies Trauma Limb Reconstr 2021;16(3):127-131.
背景:骨髓炎病原微生物的分离是临床医生面临的主要挑战。提出了几种快速准确地鉴定微生物的方法。在微生物检测和表征领域中使用拉曼光谱的兴趣越来越大。本文探讨了利用拉曼光谱鉴定引起骨髓炎的最难分离的微生物之一。方法与结果:采集全膝关节置换术患者新鲜健康人骨标本。然后用新鲜的铜绿假单胞菌(PAO)培养物接种这些样品。在6周的时间内监测细菌生长和骨超微结构的变化。实验证实溶骨PAO分泌物引起骨超微结构破坏。发现了与PAO结构细胞膜相关的拉曼特异光谱特征，表明骨表面有细菌存活。结论:本研究表明，随着时间的推移，拉曼光谱有希望识别接种骨样品表面细菌的存在。它能够检测细菌的溶骨活性以及对PAO毒力特异性的超微结构。该方法对现有的骨感染诊断方法具有辅助作用，可快速准确地鉴定骨感染细菌。Al Ghaithi A, Al Bimani A, Al Maskari S.铜绿假单胞菌生长及其对人体骨溶解的影响的体外研究。创伤肢体重建，2011;16(3):127-131。
期刊介绍：
Strategies in Trauma and Limb Reconstruction is dedicated to surgeons, allied medical professionals and researchers in the field of orthopaedics and trauma. The scope of the journal is to discuss the fields of skeletal injury, and the complications thereof, congenital and acquired limb deformities and deficiencies, and orthopaedic-related infection, together with their surgical and non-surgical treatments. The journal publishes original articles, reviews, case reports, descriptions of new or recognised treatment techniques, forum discussions of clinical scenarios and relevant correspondence. It aims to provide a widely accessible source of useful information to practitioners in the field through the problem- or technique-based approach of published articles.
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