Genes and proteins associated with ribeye area and meat tenderness in a commercial Nellore cattle population.

IF 2.3 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Genome Pub Date : 2022-04-01 Epub Date: 2021-12-03 DOI:10.1139/gen-2020-0163

Gabriela B Frezarim, Larissa F S Fonseca, Bruna M Salatta, Danielly B S Silva, Tiago Bresolin, Leonardo de Oliveira Seno, Alexéia Barufatti, Jesus Aparecido Ferro, Lucia Galvão Albuquerque

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引用次数: 2

Abstract

Despite several studies on genetic markers and differentially expressed genes related to ribeye area (REA) and tenderness traits in beef cattle, there is divergence in the results regarding the genes associated with these traits. Thirteen genes associated with or exhibiting biological functions that might influence such phenotypes were included in this study. A total of five genes for REA (IGF-1, IGF-2, MSTN, NEDD4, and UBE4A) and eight genes for meat tenderness (CAPN1, CAPN2, CAST, HSPB1, DNAJA1, FABP4, SCD, and PRKAG3) were selected from previous studies on beef cattle. Genes and their respective proteins expression were validated in a commercial population of Nellore cattle using quantitative real-time PCR (RT-qPCR) and advanced mass spectrometry (LC/MS-MS) techniques, respectively. The MSTN gene was upregulated in animals with low REA. The CAPN1, CAPN2, CAST, HSPB1, and DNAJA1 genes were upregulated in animals with tough meat. The proteins translated by these genes were not differentially expressed. Our results confirm the potential of some of the studied genes as biomarkers for carcass and meat quality traits in Nellore cattle.

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内洛尔牛商业种群中与肋眼面积和肉嫩度相关的基因和蛋白质。

尽管对肉牛肋眼面积和嫩度性状相关的遗传标记和差异表达基因进行了一些研究，但对这些性状相关基因的研究结果存在分歧。本研究包括了13个与这些表型相关或表现出可能影响这些表型的生物学功能的基因。从已有的肉牛研究中共筛选出5个REA基因(IGF-1、IGF-2、MSTN、NEDD4和UBE4A)和8个肉嫩度基因(CAPN1、CAPN2、CAST、HSPB1、DNAJA1、FABP4、SCD和PRKAG3)。利用实时荧光定量PCR (RT-qPCR)和先进质谱(LC/MS-MS)技术分别在Nellore牛的商业群体中验证了基因及其各自的蛋白表达。低REA动物的MSTN基因表达上调。在硬肉动物中，CAPN1、CAPN2、CAST、HSPB1和DNAJA1基因表达上调。这些基因翻译的蛋白没有差异表达。我们的研究结果证实了一些研究基因作为内洛牛胴体和肉质性状生物标志物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genome 生物-生物工程与应用微生物

CiteScore

5.30

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.