Plasmid Carrying bla _CTX-M-15, bla _PER-1, and bla _TEM-1 Genes in Citrobacter spp. From Regional Hospital in Mexico.

IF 4 4区医学 Q1 INFECTIOUS DISEASES Infectious Diseases Pub Date : 2022-01-18 eCollection Date: 2022-01-01 DOI:10.1177/11786337211065750

Cindy Negrete-González, Edgar Turrubiartes-Martínez, Miriam Briano-Macias, Daniel Noyola, Luis Fernando Pérez-González, Roberto González-Amaro, Perla Niño-Moreno

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引用次数: 2

Abstract

Introduction: Citrobacter spp. is an opportunistic bacteria that have been recognized as significant pathogens in patients with underlying diseases or immunocompromised status. The aim of this study was to identify extended-spectrum β-lactamases in clinical isolates of Citrobacter spp.

Methods: This cross-sectional study was conducted at Hospital Central "Dr. Ignacio Morones Prieto" in San Luis Potosi, Mexico. Nineteen isolates of Citrobacter spp. were obtained from clinical specimens between April to December 2015. Four isolates were resistant to third-generation cephalosporins. The presence of genes encoding ESBL (bla _CTX-M-15, bla _TEM-1, bla _VEB-1, bla _SHV, and bla _PER-1) was analyzed by PCR. For this purpose, plasmid DNA was extracted and horizontally transferred to recipient E. coli Top 10.

Results: bla _CTX-M-15 and bla _VEB-1 genes were detected in Citrobacter freundii and Citrobacter sedlakii, whereas bla _PER-1 gene was identified in 1 isolate of Citrobacter freundii. In contrast, bla _SHV gene was not detected in any isolate. One strain carried bla _CTX-M-15, bla _TEM-1, bla _VEB-1, and bla _PER-1 genes, most in a 275-kb plasmid.

Conclusion: This study shows the presence of different types of ESBL in clinical isolates of Citrobacter freundii and Citrobacter sedlakii, which confer resistance to broad-spectrum β-lactams. The plasmid identified in this study harboring ESBL genes could play an important role in the dissemination of antibiotic resistance.

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墨西哥地区医院柠檬酸杆菌中携带bla CTX-M-15、bla PER-1和bla TEM-1基因的质粒

柠檬酸杆菌是一种机会性细菌，已被认为是潜在疾病或免疫功能低下患者的重要病原体。本研究的目的是鉴定临床分离的柠檬酸杆菌属的广谱β-内酰胺酶。方法:本横断面研究在墨西哥圣路易斯波托西的中心医院“Dr. Ignacio Morones Prieto”进行。2015年4 - 12月从临床标本中分离到枸橼酸杆菌19株。4株对第三代头孢菌素耐药。PCR分析ESBL编码基因(bla CTX-M-15、bla TEM-1、bla VEB-1、bla SHV和bla PER-1)的存在。为此，提取质粒DNA并水平转移到受体大肠杆菌Top 10。结果:在弗氏Citrobacter freundii和Citrobacter sedlakii中检测到bla CTX-M-15和bla VEB-1基因，在1株弗氏Citrobacter freundii分离物中检测到bla PER-1基因。相比之下，bla SHV基因未在任何分离物中检测到。其中一个菌株携带bla CTX-M-15、bla TEM-1、bla VEB-1和bla PER-1基因，大部分在一个275 kb的质粒中。结论:本研究表明，临床分离的弗氏Citrobacter freundii和Citrobacter sedlakii存在不同类型的ESBL，这使得它们对广谱β-内酰胺具有耐药性。本研究中发现的含有ESBL基因的质粒可能在抗生素耐药性的传播中发挥重要作用。

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来源期刊

Infectious Diseases INFECTIOUS DISEASES-

CiteScore

8.20

自引率

1.70%

发文量

审稿时长

8 weeks

期刊介绍： Infectious Diseases (formerly Scandinavian Journal of Infectious Diseases) is a peer-reviewed journal publishing articles on all aspects of human infection, including pathogenesis, diagnosis, and treatment of infectious diseases, and also on medical microbiology and epidemiology