Halomonas faecis sp. nov., a halophilic bacterium isolated from human faeces.

Abstract

A novel moderately halophilic, Gram-stain-negative, catalase- and oxidase-positive, strictly aerobic, non-sporulating, non-motile rod, designated strain JSM 104105 ^T, was isolated from human faeces. Strain JSM 104105 ^T was able to grow with 0.5-18% (w/v) NaCl (optimum 4-9%), at pH 6-10.5 (optimum pH 7-8) and at 10-40 °C (optimum 30 °C) in complex media. The major cellular fatty acids were C_18:1ω7c, C_16:0, C_16:1ω7c and/or C_16:1ω6c, C_19:0 cyclo ω8c and C_12:0 3-OH. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid and three unidentified phospholipids. The predominant respiratory quinone was Q-9 and the genomic DNA G + C content was 64.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 104105 ^T should be assigned to the genus Halomonas, and was most closely related to Halomonas gudaonensis SL014B-69 ^T (99.0% sequence similarity), followed by Halomonas azerbaijanica TBZ202^T (98.6%) and Halomonas lysinitropha 3(2)^T (97.3%). The whole genomic analysis showed that strain JSM 104105 ^T constituted a different taxon separated from the recognized Halomonas species. Combined data from phenotypic and genotypic studies demonstrated that strain JSM 104105 ^T represents a new species of the genus Halomonas, for which the name Halomonas faecis sp. nov. is proposed. The type strain is JSM 104105 ^T (= CCTCC AB 2014160 ^T = CGMCC 1.12945 ^T = KCTC 42146 ^T).