Electrical stimulation induced Hsp70 response in C2C12 cells.

IF 3.5 4区 医学 Q2 IMMUNOLOGY Exercise Immunology Review Pub Date : 2010-01-01
Lei Wang, Yuefei Liu, Hong Jin, Jürgen M Steinacker
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Abstract

Electrical stimulation (ES) is widely used in experimental and clinical settings and shows effects on cellular response to stress; however; mechanisms underlying ES-induced effects are not thoroughly understood. We investigated the Hsp70 response in mouse myoblast derived C2C12 cells to ES at 13V in different groups (A: 12 Hz, 11 min; B: 12 Hz, 90 min; C: 100 Hz, 11 min) and harvested before ES and at 0h, Jh, 4h, 8h and 12h after ES, respectively. Control cells without ES were parallel treated to each stimulated group. Hsp70 expression was determined at protein level by quantitative Western-blot and at mRNA level by real-time PCR, respectively. ES in group A caused a modest biphasic Hsp70 response at mRNA level with a slight increase at protein level. In group B Hsp70 increased significantly (P < 0.01) at mRNA (559%) and protein level (413%), and remained elevated 12 h after ES. In group C the highest Hsp70 mRNA level (14-fold increase, P < 0.01) was observed at 4h after ES with only a moderate increase at protein level (147%, P < 0.05) at 8h after ES. Thus, ES induced distinct Hsp70 responses at both protein and mRNA level, and the characteristics of ES determined the pattern and time course of Hsp70 response in the cultured cells. ES induced Hsp70 response may serve as a common mechanism underlying diverse effects of ES and plays an important role in cellular adaptive response to ES.

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电刺激诱导C2C12细胞产生Hsp70反应。
电刺激(ES)广泛应用于实验和临床环境,并显示出细胞对应激反应的影响;然而;es诱导效应的机制尚不完全清楚。我们研究了不同组小鼠成肌细胞来源的C2C12细胞在13V下对ES的Hsp70反应(A: 12 Hz, 11 min;B: 12 Hz, 90分钟;C: 100 Hz, 11 min),分别在ES前和ES后0h, Jh, 4h, 8h和12h收获。无ES的对照细胞与各刺激组平行处理。Western-blot和real-time PCR分别检测蛋白水平和mRNA水平的Hsp70表达。A组ES在mRNA水平上引起中度双相Hsp70反应,蛋白水平上略有升高。B组Hsp70 mRNA(559%)和蛋白水平(413%)显著升高(P < 0.01),并在ES后12 h保持升高。C组Hsp70 mRNA水平在ES后4h达到最高水平(升高14倍,P < 0.01),蛋白水平在ES后8h略有升高(147%,P < 0.05)。因此,ES在蛋白和mRNA水平上诱导了不同的Hsp70反应,ES的特性决定了培养细胞中Hsp70反应的模式和时间过程。ES诱导的Hsp70反应可能是ES多种作用的共同机制,在细胞对ES的适应性反应中起重要作用。
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来源期刊
Exercise Immunology Review
Exercise Immunology Review 医学-免疫学
CiteScore
16.00
自引率
0.00%
发文量
7
期刊介绍: Exercise Immunology Review (EIR) serves as the official publication of the International Society of Exercise and Immunology and the German Society of Sports Medicine and Prevention. It is dedicated to advancing knowledge in all areas of immunology relevant to acute exercise and regular physical activity. EIR publishes review articles and papers containing new, original data along with extensive review-like discussions. Recognizing the diverse disciplines contributing to the understanding of immune function, the journal adopts an interdisciplinary approach, facilitating the dissemination of research findings from fields such as exercise sciences, medicine, immunology, physiology, behavioral science, endocrinology, pharmacology, and psychology.
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