Lei Wang, Yuefei Liu, Hong Jin, Jürgen M Steinacker
{"title":"Electrical stimulation induced Hsp70 response in C2C12 cells.","authors":"Lei Wang, Yuefei Liu, Hong Jin, Jürgen M Steinacker","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Electrical stimulation (ES) is widely used in experimental and clinical settings and shows effects on cellular response to stress; however; mechanisms underlying ES-induced effects are not thoroughly understood. We investigated the Hsp70 response in mouse myoblast derived C2C12 cells to ES at 13V in different groups (A: 12 Hz, 11 min; B: 12 Hz, 90 min; C: 100 Hz, 11 min) and harvested before ES and at 0h, Jh, 4h, 8h and 12h after ES, respectively. Control cells without ES were parallel treated to each stimulated group. Hsp70 expression was determined at protein level by quantitative Western-blot and at mRNA level by real-time PCR, respectively. ES in group A caused a modest biphasic Hsp70 response at mRNA level with a slight increase at protein level. In group B Hsp70 increased significantly (P < 0.01) at mRNA (559%) and protein level (413%), and remained elevated 12 h after ES. In group C the highest Hsp70 mRNA level (14-fold increase, P < 0.01) was observed at 4h after ES with only a moderate increase at protein level (147%, P < 0.05) at 8h after ES. Thus, ES induced distinct Hsp70 responses at both protein and mRNA level, and the characteristics of ES determined the pattern and time course of Hsp70 response in the cultured cells. ES induced Hsp70 response may serve as a common mechanism underlying diverse effects of ES and plays an important role in cellular adaptive response to ES.</p>","PeriodicalId":50468,"journal":{"name":"Exercise Immunology Review","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Exercise Immunology Review","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Electrical stimulation (ES) is widely used in experimental and clinical settings and shows effects on cellular response to stress; however; mechanisms underlying ES-induced effects are not thoroughly understood. We investigated the Hsp70 response in mouse myoblast derived C2C12 cells to ES at 13V in different groups (A: 12 Hz, 11 min; B: 12 Hz, 90 min; C: 100 Hz, 11 min) and harvested before ES and at 0h, Jh, 4h, 8h and 12h after ES, respectively. Control cells without ES were parallel treated to each stimulated group. Hsp70 expression was determined at protein level by quantitative Western-blot and at mRNA level by real-time PCR, respectively. ES in group A caused a modest biphasic Hsp70 response at mRNA level with a slight increase at protein level. In group B Hsp70 increased significantly (P < 0.01) at mRNA (559%) and protein level (413%), and remained elevated 12 h after ES. In group C the highest Hsp70 mRNA level (14-fold increase, P < 0.01) was observed at 4h after ES with only a moderate increase at protein level (147%, P < 0.05) at 8h after ES. Thus, ES induced distinct Hsp70 responses at both protein and mRNA level, and the characteristics of ES determined the pattern and time course of Hsp70 response in the cultured cells. ES induced Hsp70 response may serve as a common mechanism underlying diverse effects of ES and plays an important role in cellular adaptive response to ES.
期刊介绍:
Exercise Immunology Review (EIR) serves as the official publication of the International Society of Exercise and Immunology and the German Society of Sports Medicine and Prevention. It is dedicated to advancing knowledge in all areas of immunology relevant to acute exercise and regular physical activity. EIR publishes review articles and papers containing new, original data along with extensive review-like discussions. Recognizing the diverse disciplines contributing to the understanding of immune function, the journal adopts an interdisciplinary approach, facilitating the dissemination of research findings from fields such as exercise sciences, medicine, immunology, physiology, behavioral science, endocrinology, pharmacology, and psychology.