Preliminary Mechanistic Study on the Trachea Smooth Muscle Relaxant Activity of Aqueous Leaf Extract of Tridax Procumbens in Male Wistar Rats.

IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Pharmacopuncture Pub Date : 2022-09-30 DOI:10.3831/KPI.2022.25.3.209

Shakiru Ademola Salami, Hussein Mofomosara Salahdeen, Babatunde Shuaib Anidu, Babatunde Adekunle Murtala, AbdulRasak Akinola Alada

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Abstract

Objectives: Aqueous leaf extract of Tridax procumbens (ALETP) has potent relaxant activity. However, this relaxant activity in respiratory smooth muscle remains uninvestigated. This study investigates the effect of ALETP on the contractile activity of tracheal smooth muscle (TSM) in adult male Wistar rats.

Methods: Twelve male Wistar rats divided into 2 groups and were treated with either 100 mg/kg of ALETP (ALETP treatment group) or vehicle (distilled water; control group) through oral gavage for 4 weeks. Dose responses of TSM from the 2 groups to acetylcholine (10^-9 to 10^-5 M), phenylephrine (10^-9 to 10^-5 M), and potassium chloride (KCl; 10^-9 to 10^-4 M) were determined cumulatively. Furthermore, cumulative dose responses to acetylcholine (10^-9 to 10^-5 M) after pre-incubation of TSM with atropine (10^-5 M), L-NAME (10^-4 M), indomethacin (10^-4 M), and nifedipine (10^-4 M), were determined.

Results: Treatment with ALETP substantially inhibited TSM contraction stimulated by cumulative doses of acetylcholine, phenylephrine, and KCl. Furthermore, preincubation of TSM from the 2 groups in atropine significantly inhibited contractility in TSM. Incubation in L-NAME and indomethacin also significantly inhibited contractility in TSM of ALETP-treated rats compared to that of controls. Contractile activity of the TSM was also inhibited significantly with incubation in nifedipine in ALETP-treated rats.

Conclusion: ALETP enhanced relaxant activity in rat TSM primarily by blocking the L-type calcium channel and promoting endothelial nitric oxide release. ALETP contains agents that may be useful in disorders of the respiratory tract.

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原藜叶水提物对雄性Wistar大鼠气管平滑肌松弛作用的初步机制研究。

目的:原藜叶水提物(ALETP)具有较强的松弛活性。然而，这种在呼吸平滑肌中的松弛活性仍未被研究。本研究探讨ALETP对成年雄性Wistar大鼠气管平滑肌(TSM)收缩活动的影响。方法:雄性Wistar大鼠12只，随机分为2组，分别给予ALETP 100 mg/kg (ALETP治疗组)或载药(蒸馏水;对照组)灌胃4周。两组小鼠TSM对乙酰胆碱(10-9 ~ 10-5 M)、苯肾上腺素(10-9 ~ 10-5 M)、氯化钾(KCl;10-9 ~ 10-4 M)累积测定。进一步测定TSM与阿托品(10-5 M)、L-NAME (10-4 M)、吲哚美辛(10-4 M)、硝苯地平(10-4 M)预孵育后乙酰胆碱(10-9 ~ 10-5 M)的累积剂量反应。结果:ALETP治疗可显著抑制乙酰胆碱、苯肾上腺素和KCl累积剂量刺激的TSM收缩。此外，两组经阿托品预孵育后，TSM的收缩性明显受到抑制。与对照组相比，L-NAME和吲哚美辛也显著抑制了aletp处理大鼠的TSM收缩力。aletp处理大鼠经硝苯地平孵育后，TSM的收缩活性也明显受到抑制。结论:ALETP增强大鼠TSM松弛剂活性主要是通过阻断l型钙通道，促进内皮细胞一氧化氮释放。ALETP含有可能对呼吸道疾病有用的药物。

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来源期刊

Journal of Pharmacopuncture INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.10

自引率

7.10%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.