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Assessing Hwa-byung Vulnerability Using the Hwa-byung Personality Scale: a comparative study of machine learning approaches. 使用Hwa-byung人格量表评估Hwa-byung脆弱性:机器学习方法的比较研究。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.358
Chan-Young Kwon, Boram Lee, Sung-Hee Kim, Seok Chan Jeong, Jong-Woo Kim

Objectives: To develop and compare machine learning models to classify individuals vulnerable to Hwa-byung (HB) using an existing HB personality scale and to evaluate the efficacy of these models in predicting HB vulnerability.

Methods: We analyzed data from 500 Korean adults (aged 19-44) using HB personality and symptom scales. We used various machine learning techniques, including the random forest classifier (RFC), XGBoost classifier, logistic regression, and their ensemble method (RFC-XGC-LR). The models were developed using recursive feature elimination with cross-validation for feature selection and evaluated using multiple performance metrics, including accuracy, precision, recall, specificity, and area under the receiver operating characteristic curve (AUROC).

Results: The 16 items on the HB personality scale were identified as optimal features to predict high HB symptom scores requiring further clinical evaluation. The ensemble model slightly outperformed the other models, with an accuracy of 0.80 and an AUROC of 0.86, in the test set. Notably, item 16 ("I often feel guilty easily") of the HB personality scale showed the greatest importance in predicting HB vulnerability across all models. Although all models showed consistent performance across training, validation, and test sets, the RFC model exhibited signs of slight overfitting, with a higher AUROC of 0.97 in the training dataset compared to 0.85 in the validation and 0.86 in the test datasets.

Conclusion: Machine learning models, particularly the ensemble method, show capabilities promising for screening individuals with high HB symptom scores based on personality traits, potentially facilitating early referral for clinical evaluation. These models can improve the efficiency and accuracy of the HB risk assessment in clinical settings, potentially aiding early intervention and prevention strategies.

目的:开发和比较机器学习模型,使用现有的HB人格量表对HB易感性个体进行分类,并评估这些模型在预测HB易感性方面的功效。方法:我们使用HB人格和症状量表分析了500名韩国成年人(19-44岁)的数据。我们使用了各种机器学习技术,包括随机森林分类器(RFC)、XGBoost分类器、逻辑回归及其集成方法(RFC- xgc - lr)。这些模型采用递归特征消除和交叉验证来进行特征选择,并使用多个性能指标进行评估,包括准确性、精密度、召回率、特异性和接收者工作特征曲线下面积(AUROC)。结果:HB人格量表上的16项被确定为预测HB高症状评分的最佳特征,需要进一步的临床评估。在测试集中,集成模型的准确率为0.80,AUROC为0.86,略优于其他模型。值得注意的是,HB人格量表的第16项(“我经常很容易感到内疚”)在预测所有模型中的HB脆弱性方面显示出最大的重要性。尽管所有模型在训练集、验证集和测试集上都表现出一致的性能,但RFC模型表现出轻微的过拟合迹象,训练数据集中的AUROC为0.97,而验证数据集中的AUROC为0.85,测试数据集中的AUROC为0.86。结论:机器学习模型,特别是集成方法,显示出基于人格特征筛选HB症状得分高的个体的能力,可能有助于早期转诊进行临床评估。这些模型可以提高临床环境中HB风险评估的效率和准确性,可能有助于早期干预和预防策略。
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引用次数: 0
Effect of Herbal Medicine (Hwanglyeonhaedok-tang) on Insomnia Patients with Bedtime Procrastination: study protocol for a randomized controlled trial. 黄莲海德汤对失眠患者睡前拖延症的疗效:随机对照试验的研究方案。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.322
Yujin Choi, Pyung-Wha Kim, Hyo-Ju Park, So-Young Jung, Ojin Kwon, In Chul Jung, Hyungjun Kim, Kyung-Min Shin

Objectives: Insomnia, characterized by difficulty initiating or maintaining sleep, affects a significant portion of the global population. Bedtime procrastination, which is a voluntary delay in going to bed, is a major contributing factor to the prevalence of sleep deprivation in modern societies. Hwanglyeonhaedok-tang (HJD decoction) has shown promise in improving the symptoms of insomnia. This study aimed to evaluate the efficacy of HJD decoction in patients with insomnia and bedtime procrastination.

Methods: This study employs a parallel-group design, comparing HJD decoction to usual care in a 11 ratio. Sixty participants with insomnia and bedtime procrastination will be enrolled. The experimental group will receive HJD decoction for 4 weeks, while both groups will undergo a brief behavioral treatment for insomnia. The primary outcome will be the change in the Insomnia Severity Index score (ISI) from baseline to 4 weeks. Secondary outcomes include sleep diary metrics, the Pittsburgh Sleep Quality Index (PSQI), and Bedtime Procrastination Scale (BPS). The exploratory outcomes include perceived stress, anxiety, smartphone use, and heart rate variability.

Conclusion: This trial examines the role of herbal medicine in treating a specific type of insomnia that is increasingly common in modern society. The combination of HJD decoction with behavioral intervention offers a comprehensive approach to treating insomnia complicated by bedtime procrastination. The results will provide valuable insights into integrative treatment strategies for sleep disorders in the digital age.

目的:失眠,其特征是难以启动或维持睡眠,影响了全球人口的很大一部分。睡前拖延症,即自愿推迟上床睡觉,是现代社会普遍存在的睡眠剥夺的一个主要因素。黄莲海德汤在改善失眠症状方面表现出了希望。本研究旨在评价黄芪汤对失眠伴睡前拖延症患者的治疗效果。方法:本研究采用平行组设计,以11比1的比例比较黄芪汤与常规护理。60名患有失眠和睡前拖延症的参与者将被纳入研究。试验组给予HJD汤剂治疗4周,两组均进行简短的失眠症行为治疗。主要结果将是失眠严重程度指数评分(ISI)从基线到4周的变化。次要结果包括睡眠日记指标、匹兹堡睡眠质量指数(PSQI)和就寝拖延量表(BPS)。探索性结果包括感知压力、焦虑、智能手机使用和心率变异性。结论:这项试验检验了草药在治疗现代社会中越来越常见的一种特定类型的失眠中的作用。HJD汤剂结合行为干预治疗失眠伴睡前拖延症是一种综合性的治疗方法。研究结果将为数字时代睡眠障碍的综合治疗策略提供有价值的见解。
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引用次数: 0
Efficacy of Sophora japonica L. Pill in Subjective Memory Complaints in Healthy Adults: a protocol of randomized, double-blind, placebo-controlled, parallel-group, clinical trial. 苦参丸对健康成人主观记忆疾患的疗效:随机、双盲、安慰剂对照、平行组临床试验方案
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.349
Yunna Kim, Seung-Ho Lee, Yerim Jeon, Seung-Hun Cho

Objectives: Subjective memory complaints, increasingly common among older adults, may indicate early cognitive decline or dementia. Sophora japonica L., a herbal medicine in Korean medicine, has shown potential cognitive benefits in preclinical studies through neuroprotective and anti-inflammatory properties. Given limited efficacy of current pharmacological treatments for cognitive impairment and growing interest in natural products, investigating S. japonica extract in humans is warranted.

Methods: This randomized, double-blind, placebo-controlled trial will involve 100 participants aged 40-70 years with subjective memory complaints but without diagnosed cognitive impairment. Participants will receive either S. japonica extract (1,250 mg) or placebo daily for 8 weeks. The primary outcome is change in digit span test score, assessing short-term memory and attention. Secondary outcomes include changes in other cognitive function tests (visual short-term memory, visual learning, and verbal learning), as well as measures of depression (Beck Depression Inventory-II), anxiety (State-Trait Anxiety Inventory-Y), stress (Stress Response Inventory), heart rate variability, and quality of life (EuroQol 5-Dimension-3L). Assessments will be conducted at baseline and after 8 weeks, with safety monitoring throughout the study period.

Conclusion: This study will provide evidence on the efficacy and safety of S. japonica extract for improving cognitive function in adults with subjective memory complaints. If proven effective, this supplement could offer a new approach for supporting cognitive health in aging populations. The comprehensive assessment of cognitive, mood, and quality of life outcomes will allow thorough evaluation of its potential benefits.

目的:主观记忆抱怨在老年人中越来越普遍,可能预示着早期认知能力下降或痴呆。韩国医学中草药苦参具有神经保护和抗炎作用,在临床前研究中显示出可能的认知益处。鉴于目前对认知障碍的药物治疗效果有限,以及人们对天然产物的兴趣日益浓厚,研究粳稻提取物在人体中的作用是有必要的。方法:这项随机、双盲、安慰剂对照试验将纳入100名年龄在40-70岁之间的参与者,他们有主观记忆障碍,但没有诊断出认知障碍。参与者每天接受粳稻提取物(1,250毫克)或安慰剂,持续8周。主要结果是数字广度测试分数的变化,评估短期记忆和注意力。次要结果包括其他认知功能测试(视觉短期记忆、视觉学习和语言学习)的变化,以及抑郁(贝克抑郁量表- ii)、焦虑(状态-特质焦虑量表- y)、压力(应激反应量表)、心率变异性和生活质量(EuroQol 5维- 3l)的测量。评估将在基线和8周后进行,并在整个研究期间进行安全性监测。结论:本研究为粳米提取物改善成人主观记忆疾患认知功能的有效性和安全性提供了依据。如果证明有效,这种补充剂可以为支持老年人的认知健康提供一种新的方法。对认知、情绪和生活质量结果的综合评估将允许对其潜在益处进行彻底的评估。
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引用次数: 0
Cannabigerol Treatment Shows Antiproliferative Activity and Causes Apoptosis of Human Colorectal Cancer Cells. 大麻酚治疗显示抗增殖活性并引起人结直肠癌细胞凋亡。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.332
Ju-Hee Park, Yu-Na Hwang, Han-Heom Na, Do-Yeon Kim, Hyo-Jun Lee, Tae-Hyung Kwon, Jin-Sung Park, Keun-Cheol Kim

Objectives: To determine growth inhibitory and anti-cancer effects of Cannabigerol (CBG) in human colorectal cancer cells.

Methods: Anti-proliferative effect of CBG was examined using MTT assay and two colorectal cancer cells (SW480 and LoVo cells). Cell death ratio was analyzed using Annexin V/PI staining experiment. Cell cycle distribution was analyzed using flow cytometry. We also performed western blot analysis on apoptotic marker proteins.

Results: CBG showed growth inhibitory effect in colorectal cancer cells using MTT assay. IC50 concentration of CBG was 34.89 μM in SW480 cells and 23.51 μM in LoVo cells. Annexin V/PI staining showed that CBG treatment increased apoptotic cells from 4.8% to 31.7% in SW480 cells and from 7.7% to 33.9% in LoVo cells. Flow cytometry confirmed that CBG increased sub G1 population via G1 arrest in both SW480 and LoVo cells. Western blot analysis showed that CBG increased expression levels of cell death-related proteins such as cleaved PARP-1, cleaved caspase 9, p53, and caspase 3.

Conclusion: CBG treatment shows antiproliferative activity and causes apoptosis of colorectal cancer cells, suggesting that CBG is applicable as a promising anticancer drug.

目的:研究大麻酚(Cannabigerol, CBG)对人大肠癌细胞的生长抑制和抗癌作用。方法:采用MTT法和两种结直肠癌细胞(SW480和LoVo细胞)检测CBG的抗增殖作用。采用Annexin V/PI染色法分析细胞死亡率。流式细胞术分析细胞周期分布。我们还对凋亡标记蛋白进行了western blot分析。结果:MTT法测定CBG对结直肠癌细胞有抑制生长的作用。CBG在SW480细胞中的IC50浓度为34.89 μM,在LoVo细胞中的IC50浓度为23.51 μM。Annexin V/PI染色显示,CBG处理使SW480细胞的凋亡细胞从4.8%增加到31.7%,使LoVo细胞的凋亡细胞从7.7%增加到33.9%。流式细胞术证实CBG在SW480和LoVo细胞中通过G1阻滞增加了亚G1群。Western blot分析显示,CBG增加了细胞死亡相关蛋白如cleaved PARP-1、cleaved caspase 9、p53和caspase 3的表达水平。结论:CBG对结直肠癌具有抗增殖作用,可引起结直肠癌细胞凋亡,提示CBG是一种有前景的抗癌药物。
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引用次数: 0
Investigating the Impact of Sumac Capsules on Postpartum Bleeding among Women at Risk of Bleeding: a three-blind randomized clinical trial. 研究漆树胶囊对出血风险妇女产后出血的影响:一项三盲随机临床试验。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.287
Mahsa Payranj, Maryam Moradi, Hassan Rakhshandeh, Vahid Ghavami, Seyed Mostafa Moshirian Farahi, Maliheh Gharaee Najafabadi, Morvarid Irani, Azin Niazi

Objectives: Postpartum hemorrhage is a leading cause of maternal mortality worldwide. Emerging evidence suggests that the sumac plant possesses astringent and anti-inflammatory properties that may help reduce menstrual bleeding. Therefore, this study aimed to determine the effect of sumac capsules on postpartum bleeding among women at risk of excessive bleeding.

Methods: This randomized, triple-blind clinical trial was conducted in 2022-2023. Participants were 72 women who were referred for vaginal delivery to the 9th Day Hospital in Torbat Heydariyeh city, Iran. Participants were randomly divided into two groups immediately after delivery, the placebo group received routine care. The intervention group, in addition to routine care, received two oral capsules of 500 mg sumac immediately after the expulsion of the placenta and fetal membranes, as well as two hours later. The amount of bleeding was measured using plastic bags for blood collection, which were weighed along with the sheets and pads used at hours 1, 2, 3, and 4 postpartum. Data analysis was performed using SPSS version 27, and statistical significance was set at p < 0.05. 65 women completed the study.

Results: The sumac group exhibited lower mean bleeding volumes than the placebo group at all measured time points (first, second, third, and fourth hours after delivery). The independent t-test analysis revealed significant differences between the two groups' bleeding volume at the end of the first hour (p = 0.013), second hour (p < 0.001), third hour (p < 0.001), and fourth hour (p < 0.001); that was less in sumac group. Overall, the sumac group demonstrated a significantly lower mean total bleeding volume in the first four hours after delivery (215.78 ± 16.92 cc) compared to the placebo group (261.51 ± 17.258 cc) (p < 0.001).

Conclusion: Sumac capsules can be considered as a potentially effective and safe intervention for reducing postpartum bleeding among women at risk.

目的:产后出血是全世界孕产妇死亡的主要原因。新出现的证据表明,漆树植物具有收敛和抗炎的特性,可能有助于减少月经出血。因此,本研究旨在确定漆树胶囊对有出血过多风险妇女产后出血的影响。方法:该随机、三盲临床试验于2022-2023年进行。参与者是72名妇女,她们被转诊到伊朗Torbat Heydariyeh市的第九天医院进行阴道分娩。参与者在分娩后立即随机分为两组,安慰剂组接受常规护理。干预组在常规护理的基础上,在胎盘和胎膜排出后立即和2小时后口服两粒500毫克漆树甙胶囊。在产后1、2、3和4小时,用塑料袋和床单、卫生巾一起称重,用于采血,测量出血量。数据分析采用SPSS 27版,p < 0.05为差异有统计学意义。65名女性完成了这项研究。结果:漆树组在所有测量时间点(分娩后第一、第二、第三和第四小时)的平均出血量均低于安慰剂组。独立t检验分析显示,两组患者第1小时(p = 0.013)、第2小时(p < 0.001)、第3小时(p < 0.001)、第4小时出血量差异有统计学意义(p < 0.001);这在漆树组较少。总体而言,与安慰剂组(261.51±17.258 cc)相比,漆树组在分娩后4小时的平均总出血量(215.78±16.92 cc)显着降低(p < 0.001)。结论:漆树胶囊可以被认为是一种潜在的有效和安全的干预措施,以减少产后出血的危险妇女。
{"title":"Investigating the Impact of Sumac Capsules on Postpartum Bleeding among Women at Risk of Bleeding: a three-blind randomized clinical trial.","authors":"Mahsa Payranj, Maryam Moradi, Hassan Rakhshandeh, Vahid Ghavami, Seyed Mostafa Moshirian Farahi, Maliheh Gharaee Najafabadi, Morvarid Irani, Azin Niazi","doi":"10.3831/KPI.2024.27.4.287","DOIUrl":"10.3831/KPI.2024.27.4.287","url":null,"abstract":"<p><strong>Objectives: </strong>Postpartum hemorrhage is a leading cause of maternal mortality worldwide. Emerging evidence suggests that the sumac plant possesses astringent and anti-inflammatory properties that may help reduce menstrual bleeding. Therefore, this study aimed to determine the effect of sumac capsules on postpartum bleeding among women at risk of excessive bleeding.</p><p><strong>Methods: </strong>This randomized, triple-blind clinical trial was conducted in 2022-2023. Participants were 72 women who were referred for vaginal delivery to the 9th Day Hospital in Torbat Heydariyeh city, Iran. Participants were randomly divided into two groups immediately after delivery, the placebo group received routine care. The intervention group, in addition to routine care, received two oral capsules of 500 mg sumac immediately after the expulsion of the placenta and fetal membranes, as well as two hours later. The amount of bleeding was measured using plastic bags for blood collection, which were weighed along with the sheets and pads used at hours 1, 2, 3, and 4 postpartum. Data analysis was performed using SPSS version 27, and statistical significance was set at p < 0.05. 65 women completed the study.</p><p><strong>Results: </strong>The sumac group exhibited lower mean bleeding volumes than the placebo group at all measured time points (first, second, third, and fourth hours after delivery). The independent t-test analysis revealed significant differences between the two groups' bleeding volume at the end of the first hour (p = 0.013), second hour (p < 0.001), third hour (p < 0.001), and fourth hour (p < 0.001); that was less in sumac group. Overall, the sumac group demonstrated a significantly lower mean total bleeding volume in the first four hours after delivery (215.78 ± 16.92 cc) compared to the placebo group (261.51 ± 17.258 cc) (p < 0.001).</p><p><strong>Conclusion: </strong>Sumac capsules can be considered as a potentially effective and safe intervention for reducing postpartum bleeding among women at risk.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 4","pages":"287-296"},"PeriodicalIF":1.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Evaluation of the Preventive Effects of Citral, Silymarin, Thymoquinone, and Curcumin on 5-Fluorouracil-Induced Cardiac and Pulmonary Toxicity in Rats. 柠檬醛、水飞蓟素、百里醌和姜黄素对5-氟尿嘧啶致大鼠心肺毒性预防作用的比较评价。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.277
Tohid Zahed, Nasrollah Ahmadi, Mohammad Foad Noorbakhsh, Nazifi Saeed, Oryan Ahmad

Objectives: 5-fluorouracil chemotherapy is a highly recommended treatment for different types of solid tumors. However, this treatment can have severe side effects on the heart and lungs. In this study, we compared the protective effects of citral, silymarin, thymoquinone, and curcumin against 5-fluorouracil-induced toxicity in the heart and lungs of rats.

Methods: 56 healthy adult male rats were randomly assigned to seven experimental groups (n = 8), including healthy and carrier (dimethylsulfoxide) groups, 5-fluorouracil, citral group, silymarin group, thymoquinone group, and curcumin group. Blood samples and representative tissue specimens of the heart and lungs were immediately collected at the end of the experiment to measure the biochemical parameters, conduct histopathological studies, and analyze antioxidant activity, respectively.

Results: The intraperitoneal administration of 5-fluorouracil caused cardiotoxicity, as evidenced by elevated serum levels of creatine phosphokinase, creatine kinase-MB (p < 0.05), and lactate dehydrogenase (p < 0.05). Besides, 5-fluorouracil increased malondealdehyde levels, indicating a lipid peroxidation index and a decrease in the total antioxidant capacity index in the cardiac and pulmonary tissues (p < 0.05) of the animals. The preventive and therapeutic use of all the above compounds, in combination with 5-fluorouracil, led to a decrease in the mentioned cardiac serum biomarkers and malondealdehyde in the animals (p < 0.05). In addition, all the therapeutic compounds increased total antioxidant capacity in the heart and lungs (p < 0.05), indicating a high antioxidant capacity of these biological substances in ameliorating the resultant oxidative and histologic damages.

Conclusion: Our study indicated that the natural compounds citral, silymarin, thymoquinone, and curcumin, when combined with 5-fluorouracil, could minimize the histopathological and biochemical changes caused by 5-fluorouracil treatment in the heart and pulmonary tissues likely via antioxidant mechanisms. These products can be useful and effective in chemotherapy patients by reducing the potential adverse effects of 5-fluorouracil administration.

目的:5-氟尿嘧啶化疗是一种强烈推荐的治疗不同类型的实体瘤。然而,这种治疗对心脏和肺部有严重的副作用。在本研究中,我们比较了柠檬醛、水飞蓟素、百里醌和姜黄素对5-氟尿嘧啶引起的大鼠心脏和肺部毒性的保护作用。方法:选取健康成年雄性大鼠56只,随机分为健康及载体(二甲亚砜)组、5-氟尿嘧啶组、柠檬醛组、水飞蓟素组、百里醌组、姜黄素组7个实验组(n = 8)。实验结束后立即采集心脏和肺部代表性组织标本,分别进行生化参数测定、组织病理学研究和抗氧化活性分析。结果:5-氟尿嘧啶腹腔注射引起心肌毒性,血清肌酸磷酸激酶、肌酸激酶- mb水平升高(p < 0.05),乳酸脱氢酶水平升高(p < 0.05)。此外,5-氟尿嘧啶增加了丙二醛水平,导致动物心肺组织脂质过氧化指数和总抗氧化能力指数降低(p < 0.05)。将上述所有化合物与5-氟尿嘧啶联合用于预防和治疗,可导致上述动物心脏血清生物标志物和丙二醛的降低(p < 0.05)。此外,所有治疗性化合物均增加了心脏和肺部的总抗氧化能力(p < 0.05),表明这些生物物质具有较高的抗氧化能力,可以改善由此引起的氧化和组织损伤。结论:我们的研究表明,天然化合物柠檬醛、水飞蓟素、百里醌和姜黄素与5-氟尿嘧啶联用时,可能通过抗氧化机制减少5-氟尿嘧啶治疗引起的心肺组织病理和生化变化。通过减少5-氟尿嘧啶给药的潜在不良反应,这些产品对化疗患者是有用和有效的。
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引用次数: 0
In Vitro Assays for the Assessment of Safety and Toxicity in Pharmacopuncture Derived from Animal. 动物源性药物穿刺安全性和毒性评价的体外试验。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.308
Yu-Jin So, Hyein Jeong, Kyeong Han Kim, Seong-Gyu Ko

Objectives: Among the various treatment methods involving the use of natural substances, pharmacopuncture using animal venom is a relatively new form of acupuncture that has been developed in South Korea and is gaining popularity worldwide. Pharmacopuncture with animal venom is widely used in clinical practice; therefore, ensuring its procedural safety is crucial. This study aimed to evaluate the safety and toxicity of pharmacopuncture using animal venom.

Methods: In October 2021, nine samples of animal venom-derived pharmacopuncture products were randomly collected from four External Herbal Dispensaries (EHDs). These samples underwent sterility and microbial limit testing to ensure they were free from microbial contamination. Toxicity tests were conducted using three different cell lines to evaluate cytotoxic effects.

Results: The sterility and microbial limit tests showed no microbial growth in any of the pharmacopuncture samples. However, the toxicity tests revealed that bee venom exhibited strong cytotoxicity. Furthermore, samples containing Bovis Calculus, Fel Ursi, and Moschus also demonstrated varying degrees of cytotoxic effects.

Conclusion: This study is the first to analyze the safety and toxicity of animal venom-derived pharmacopuncture products, providing evidence for its procedural safety. Although the samples analyzed were limited to four EHDs, these findings highlight the importance of further research on the safety and toxicity of pharmacopuncture to ensure its clinical application is both effective and safe.

目的:在涉及使用天然物质的各种治疗方法中,使用动物毒液的药物穿刺是一种相对较新的针灸形式,已在韩国开发并在世界范围内越来越受欢迎。动物毒液穿刺在临床中应用广泛;因此,确保其程序安全至关重要。本研究旨在评价动物毒液穿刺的安全性和毒性。方法:于2021年10月,随机抽取4家外用中药房的动物毒液衍生药物穿针术产品9份。这些样品经过无菌和微生物限度测试,以确保它们没有微生物污染。使用三种不同的细胞系进行毒性试验以评估细胞毒性作用。结果:无菌检查和微生物限度检查均未见微生物生长。然而,毒性试验显示蜂毒表现出很强的细胞毒性。此外,含有牛牙石、熊牙石和麝香草的样品也表现出不同程度的细胞毒性作用。结论:本研究首次分析了动物毒液源性药物穿刺产品的安全性和毒性,为其程序安全性提供了依据。虽然分析的样本仅限于4个ehd,但这些发现强调了进一步研究药物穿刺安全性和毒性的重要性,以确保其临床应用既有效又安全。
{"title":"In Vitro Assays for the Assessment of Safety and Toxicity in Pharmacopuncture Derived from Animal.","authors":"Yu-Jin So, Hyein Jeong, Kyeong Han Kim, Seong-Gyu Ko","doi":"10.3831/KPI.2024.27.4.308","DOIUrl":"10.3831/KPI.2024.27.4.308","url":null,"abstract":"<p><strong>Objectives: </strong>Among the various treatment methods involving the use of natural substances, pharmacopuncture using animal venom is a relatively new form of acupuncture that has been developed in South Korea and is gaining popularity worldwide. Pharmacopuncture with animal venom is widely used in clinical practice; therefore, ensuring its procedural safety is crucial. This study aimed to evaluate the safety and toxicity of pharmacopuncture using animal venom.</p><p><strong>Methods: </strong>In October 2021, nine samples of animal venom-derived pharmacopuncture products were randomly collected from four External Herbal Dispensaries (EHDs). These samples underwent sterility and microbial limit testing to ensure they were free from microbial contamination. Toxicity tests were conducted using three different cell lines to evaluate cytotoxic effects.</p><p><strong>Results: </strong>The sterility and microbial limit tests showed no microbial growth in any of the pharmacopuncture samples. However, the toxicity tests revealed that bee venom exhibited strong cytotoxicity. Furthermore, samples containing Bovis Calculus, Fel Ursi, and Moschus also demonstrated varying degrees of cytotoxic effects.</p><p><strong>Conclusion: </strong>This study is the first to analyze the safety and toxicity of animal venom-derived pharmacopuncture products, providing evidence for its procedural safety. Although the samples analyzed were limited to four EHDs, these findings highlight the importance of further research on the safety and toxicity of pharmacopuncture to ensure its clinical application is both effective and safe.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 4","pages":"308-321"},"PeriodicalIF":1.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anthelmintic Potential of Discarded Litchi chinensis Seeds: a sustainable approach to agricultural by-product utilization. 废弃荔枝种子的驱虫潜力:农业副产品可持续利用途径。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.340
Mustakin Ahmed Shohel, Md Humayan Kabir, Sumaiya Siddika, Sheikh Arafat Rahman, Nirmal Chandra Mahat, Tarique Muhammad Tawabul Islam, Md Rakibul Hasan Rahat, Ivvala Anand Shaker, Md Abul Kashem Tang

Objectives: Litchi chinensis (Sonn.), belonging to the Sapindaceae family, has historically been used for the treatment of gastrointestinal ailments, including ulcers, gastritis, diarrhea, and infections. Plants in the Sapindaceae family have demonstrated potential anthelmintic effects, while the efficacy of L. chinensis remains barely investigated. L. chinensis seeds are often discarded as waste; however, utilizing these seeds promotes sustainable practices and may provide a natural alternative to conventional anthelmintics. The study aims to investigate the qualitative phytochemicals and evaluate the anthelmintic efficacy of L. chinensis seed ethanolic extract (LCSE).

Methods: Fresh L. chinensis fruits were collected from a local market, peeled fruits and removed aril from the seeds and then washed, air-dried and extracted with ethanol. A qualitative phytochemical screening and assessment of the anthelmintic properties of LCSE were conducted using standard procedures. The time required for paralysis and death of adult earthworms (Eisenia fetida) was assessed by analyzing each test solution at five distinct dosages (5, 25, 50, 75, and 100 mg/mL). Albendazole served as the standard, while distilled water functioned as the control. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test in GraphPad Prism version 10.1.2.

Results: Qualitative analysis revealed that LCSE is rich in phytochemicals, including alkaloids, anthraquinones, carbohydrates, flavonoid, glycosides, proteins and amino acids, phenols, terpenoids, and tannins. The anthelmintic activities of the LCSE and albendazole were dose-dependent, as the time required for paralysis and mortality decreased in proportion to the concentrations increased. LCSE exhibited strong anthelmintic efficacy at a concentration of 100 mg/mL (paralysis 12.67 ± 0.33, death 14 ± 0.58), which was significantly greater than that of albendazole (paralysis 15 ± 1.15, death 17.67 ± 1.15).

Conclusion: This finding suggests that LCSE holds potent anthelmintic properties, making it a promising natural alternative to conventional treatments like albendazole.

目的:荔枝(Sonn.),属于皂角科,历史上被用于治疗胃肠道疾病,包括溃疡,胃炎,腹泻和感染。在sapinaceae家族的植物已经证明了潜在的驱虫药作用,而L. chinensis的功效还很少被研究。羊草种子常被当作废物丢弃;然而,利用这些种子促进了可持续的做法,并可能提供传统驱虫药的天然替代品。本研究旨在研究羊草种子乙醇提取物(LCSE)的定性化学成分,并对其驱虫效果进行评价。方法:从当地市场采集新鲜羊草果实,去皮、去假种皮,清洗、风干、乙醇提取。采用标准程序对LCSE的驱虫特性进行了定性的植物化学筛选和评价。通过分析五种不同剂量(5、25、50、75和100 mg/mL)的试验溶液,评估成年蚯蚓(Eisenia fetida)瘫痪和死亡所需的时间。阿苯达唑为标准品,蒸馏水为对照品。采用GraphPad Prism 10.1.2版本的双因素方差分析和Tukey多重比较检验进行统计分析。结果:定性分析表明,LCSE含有丰富的植物化学物质,包括生物碱、蒽醌类、碳水化合物、类黄酮、苷类、蛋白质和氨基酸、酚类、萜类和单宁。LCSE和阿苯达唑的驱虫活性呈剂量依赖性,麻痹所需时间和死亡率随浓度的增加而成比例降低。LCSE在浓度为100 mg/mL时表现出较强的驱虫效果(麻痹12.67±0.33,死亡14±0.58),显著高于阿苯达唑(麻痹15±1.15,死亡17.67±1.15)。结论:这一发现表明LCSE具有有效的驱虫特性,使其成为阿苯达唑等传统治疗方法的有希望的天然替代品。
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引用次数: 0
Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers. 透明胡椒通过调节心电图和心脏生物标志物对多柔比星诱导的Wistar大鼠心脏毒性的保护作用。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-12-31 DOI: 10.3831/KPI.2024.27.4.297
Efiok Aniekan Archibong, Justin Atiang Beshel, Idara Asuquo Okon, Glory Aidam Ikum, Stella Chiamaka Anaba, Daniel Udofia Owu

Objectives: This study assessed the electrocardiographic pattern and cardiac inflammatory response of doxorubicin-induced myocardial injury in Wistar rats treated with Peperomia pellucida ethanol extract.

Methods: Female Wistar rats (190-200 g) were assigned into five groups of seven rats each. The Group 1 (Control group) was given rat chow and drinking water while the Group 2 (doxorubicin group) received intraperitoneal administration of doxorubicin (2 mg/kg) once weekly for three weeks. The Group 3 (Peperomia pellucida group) received 200 mg/kg of ethanolic extract of Peperomia pellucida daily. Group 4 (Doxorubicin + P. pellucida group) received doxorubicin in addition to Peperomia pellucida. Group 5 (Captopril (50 mg/kg) was administered to another group in addition to P. pellucida while the doxorubicin + captopril group was administered captopril in addition to doxorubicin. Electrical recording and cardiac markers were evaluated.

Results: The results revealed a significant (p < 0.01) elevation of T-wave and altered electrocardiographic parameters in the doxorubicin group than the control, P. pellucida, and other experimental groups. The heart rate, cardiac troponin level, lactate dehydrogenase, creatine kinase, angiotensin-converting enzyme activities, and inflammatory biomarkers were significantly (p < 0.01) higher while nitric oxide level was significantly (p < 0.05) reduced in the doxorubicin-only group compared to the control. Cardiac cell hypertrophy and inflammatory cell infiltration were observed due to doxorubicin administration. Treatment with P. pellucida extract and captopril reversed these trends and improved the antioxidants and inflammatory activities.

Conclusion: Peperomia pellucida extract improves electrocardiographic pattern, has cardioprotective ability, and prevents doxorubicin-induced myocardial injury probably due to its phytochemical constituents and anti-inflammatory properties.

目的:观察多柔比星致Wistar大鼠心肌损伤后多柔比星乙醇提取物对心肌损伤的影响。方法:雌性Wistar大鼠(190 ~ 200 g)分为5组,每组7只。1组(对照组)给予大鼠饲料和饮水,2组(阿霉素组)给予阿霉素(2 mg/kg)腹腔注射,每周1次,连用3周。3组(透明胡椒组)每日给予透明胡椒乙醇提取物200 mg/kg。第4组(阿霉素+透明紫癜组)在给予阿霉素治疗的同时给予透明紫癜治疗。第5组(卡托普利(50 mg/kg))在加药的基础上加药,阿霉素+卡托普利组在加药的基础上加药卡托普利。评估电记录和心脏指标。结果:阿霉素组t波升高,心电图参数改变明显(p < 0.01),明显高于对照组、透明藻组和其他实验组。与对照组相比,阿霉素组的心率、心肌肌钙蛋白水平、乳酸脱氢酶、肌酸激酶、血管紧张素转换酶活性和炎症生物标志物均显著(p < 0.01)升高,一氧化氮水平显著(p < 0.05)降低。阿霉素给药后心肌细胞肥大,炎症细胞浸润。用透明叶提取物和卡托普利治疗逆转了这些趋势,并改善了抗氧化剂和炎症活性。结论:透明胡椒提取物可能与其植物化学成分及抗炎作用有关,可改善心电图,具有保护心脏的作用,并可预防阿霉素所致的心肌损伤。
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引用次数: 0
Seborrheic Dermatitis Treatment Using a Standardized Medical Insurance-Approved Korean Medicine: a case report. 使用经医疗保险批准的标准化韩药治疗脂溢性皮炎:病例报告。
IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2024-09-30 DOI: 10.3831/KPI.2024.27.3.264
Yeonkyoung Pak, Sangwon Shin

Background: Conventional treatments for seborrheic dermatitis often lead to a recurring cycle of symptom improvement and worsening, resulting in chronic conditions. Thus, safer and more effective alternatives are needed. In Korean medicine, Hwangryunhaedok-tang tablets, targeted at treating the fire-heat syndrome, offer a more fundamental approach to manage seborrheic dermatitis.

Clinical features and outcomes: In this study, we monitored the changes in the symptoms of two patients with seborrheic dermatitis who were treated with Hwangryunhaedok-tang tablets. The patients were administered this medication during the treatment period. The effectiveness of the treatment was assessed by visually recording changes in the affected skin areas using photographs and evaluating symptoms such as heat, itching, and stinging in these areas using a visual analog scale (VAS). Visible improvements in the patients' skin conditions were observed after taking Hwangryunhaedok-tang tablets. Following treatment, VAS scores for subjective symptoms such as heat sensation, itching, and stinging in the affected areas decreased.

Conclusion: This study offers evidence of a potential alternative approach for treating seborrheic dermatitis using Kyungbang Hwangryunhaedok-tang tablets. However, it highlights the necessity for further research on the appropriate dosage, side effects, and long-term effectiveness of this treatment.

背景:脂溢性皮炎的传统治疗方法往往会导致症状改善和恶化的反复循环,造成慢性疾病。因此,需要更安全、更有效的替代疗法。在韩国医学中,以治疗火热综合征为目标的黄连海德堂片为脂溢性皮炎的治疗提供了一种更根本的方法:在这项研究中,我们监测了两名接受黄莲海德堂片治疗的脂溢性皮炎患者的症状变化。患者在治疗期间服用了这种药物。通过使用照片直观记录受影响皮肤区域的变化,并使用视觉模拟量表(VAS)评估这些区域的发热、瘙痒和刺痛等症状,从而评估治疗效果。服用黄连海独汤片后,患者的皮肤状况明显改善。治疗后,患者患处的热感、瘙痒和刺痛等主观症状的 VAS 评分均有所下降:这项研究证明,使用庆邦黄莲海德堂片治疗脂溢性皮炎是一种潜在的替代方法。不过,该研究强调了进一步研究这种疗法的适当剂量、副作用和长期疗效的必要性。
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引用次数: 0
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Journal of Pharmacopuncture
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