Oxicam-type nonsteroidal anti-inflammatory drugs enhance Agrobacterium-mediated transient transformation in plants.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-09-25 DOI:10.5511/plantbiotechnology.22.0312a

Seung-Won Choi, Kie Kumaishi, Reiko Motohashi, Harumi Enoki, Wiluk Chacuttayapong, Tadashi Takamizo, Hiroaki Saika, Masaki Endo, Tetsuya Yamada, Aya Hirose, Nobuya Koizuka, Seisuke Kimura, Yaichi Kawakatsu, Hiroyuki Koga, Emi Ito, Ken Shirasu, Yasunori Ichihashi

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Abstract

Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of Agrobacterium-mediated transient transformation. TNX treatment increased the transformation efficiency of Agrobacterium-mediated transformation of Arabidopsis thaliana mature leaves by agroinfiltration. The increase of efficiency by TNX treatment was not observed in dde2/ein2/pad4/sid2 quadruple mutant, indicating that TNX inhibits the immune system mediated by jasmonic acid, ethylene, and salicylic acid against to Agrobacterium. We also found that TNX-treatment is applicable for the transient expression and subcellular localization analysis of fluorescent-tagged proteins in Arabidopsis leaf cells. In addition, we found that TNX increases the efficiency of Agrobacterium-mediated transient transformation of Jatropha. Given that treatment with oxicam compounds is a simple and cost effective method, our findings will provide a new option to overcome limitations associated with Agrobacterium-mediated transformation of various plant species.

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奥昔康类非甾体抗炎药增强农杆菌介导的植物瞬时转化。

农杆菌介导的转化是植物育种的一项重要创新，在基础研究和应用生物学中得到了广泛应用。然而，转换效率往往是该技术的限制因素。在本研究中，我们发现奥昔康型非甾体抗炎药，包括替诺昔康(TNX)，可以提高农杆菌介导的瞬时转化效率。TNX处理提高了农杆菌介导的拟南芥成熟叶片经农渗透转化的效率。在dde2/ein2/pad4/sid2四重突变体中未观察到TNX处理效率的提高，表明TNX抑制了茉莉酸、乙烯和水杨酸介导的免疫系统对农杆菌的抗性。我们还发现tnx处理适用于拟南芥叶片细胞荧光标记蛋白的瞬时表达和亚细胞定位分析。此外，我们发现TNX提高了农杆菌介导的麻疯树瞬时转化效率。鉴于使用奥昔康化合物治疗是一种简单且成本有效的方法，我们的研究结果将为克服农杆菌介导的各种植物转化相关的局限性提供一种新的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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