Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma.

IF 0.7 Q4 OTORHINOLARYNGOLOGY Turkish Archives of Otorhinolaryngology Pub Date : 2022-09-01 Epub Date: 2022-11-15 DOI:10.4274/tao.2022.2022-6-17
Çağlar Eker, Hakkı Caner İnan, Asuman Çelebi, Emine Deniz Gözen, Emin Karaman
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引用次数: 1

Abstract

Objective: Despite all the recent advancements, larynx cancer has shown no improvement in survival rates. The aim of this study was to investigate the expressions of toll-like receptor (TLR)-2, -3, and -4 genes, and determine any relationships with the histopathologic characteristics of the disease.

Methods: This retrospective study included 50 subjects who underwent total or partial laryngectomy with an open surgical method for larynx squamous cell carcinoma. Measurements of TLRs-2, -3, and -4 expression values were taken with quantitative real time-polymerase chain reaction in normal tissue and tumor tissue samples of the patients.

Results: Evaluations were made of TLR-2, -3, and -4 mRNA expressions according to 2-ΔΔCT calculations in 50 subjects with larynx cancer. When the tumor tissue was compared with the healthy tissue from the same subjects, reductions were determined in TLR expression in 86%, 84%, and 82%, respectively. This reduction in each gene expression was statistically significant (p<0.001). No statistically significant correlation was determined between the change in TLR-2, -3, and -4 expression and the histopathologic characteristics of the disease.

Conclusion: The data obtained in this study demonstrated that TLR-2, -3, and -4 expressions were reduced in larynx squamous cell cancer. The results of further studies targeting these genes would be useful in the diagnosis and treatment of the disease.

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toll样受体-2、-3和-4基因在喉鳞状细胞癌中的表达研究
目的:尽管近年来喉癌的治疗取得了很大进展,但生存率并没有提高。本研究的目的是研究toll样受体(TLR)-2、-3和-4基因的表达,并确定其与疾病组织病理特征的关系。方法:本回顾性研究包括50例接受喉鳞癌全切除或部分切除手术的患者。采用实时定量聚合酶链反应法测定患者正常组织和肿瘤组织标本中TLRs-2、-3和-4的表达值。结果:按2-ΔΔCT计算50例喉癌患者TLR-2、-3、-4 mRNA表达。当肿瘤组织与来自同一受试者的健康组织进行比较时,TLR表达分别降低了86%,84%和82%。结论:本研究获得的数据表明,TLR-2、-3、-4在喉癌中表达降低。针对这些基因的进一步研究结果将有助于该病的诊断和治疗。
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