LaeA regulates morphological development and ochratoxin A biosynthesis in Aspergillus niger.

IF 2.6 4区医学 Q2 MYCOLOGY Mycotoxin Research Pub Date : 2022-11-01 Epub Date: 2022-07-26 DOI:10.1007/s12550-022-00463-1

Mengwei Zhang, Yan Yang, Linlin Li, Shuwen Liu, Xianli Xue, Qiang Gao, Depei Wang, Ying Zhang, Jian Zhang

{"title":"LaeA regulates morphological development and ochratoxin A biosynthesis in Aspergillus niger.","authors":"Mengwei Zhang, Yan Yang, Linlin Li, Shuwen Liu, Xianli Xue, Qiang Gao, Depei Wang, Ying Zhang, Jian Zhang","doi":"10.1007/s12550-022-00463-1","DOIUrl":null,"url":null,"abstract":"<p><p>The global regulator LaeA and its orthologs govern the morphogenetic development and secondary metabolism of several filamentous ascomycetes. In Aspergillus niger, it has been shown that an LaeA ortholog (AnLaeA) regulates the production of citric acid and secondary metabolites. In this work, we constructed AnlaeA disruption and overexpression strains to investigate the roles of AnLaeA in morphological development and ochratoxin A (OTA) biosynthesis in A. niger. Phenotypic observation, chemical analysis, and gene expression analysis indicated that AnLaeA acts as a negative regulator of conidial morphogenesis and positively regulates gene expression of the OTA cluster in A. niger grown in CYA medium. However, it was observed that the upregulation of gene expression of the OTA cluster does not necessarily increase OTA production. Our results contribute to a better understanding of the AnlaeA regulatory mechanism and suggest the AnlaeA gene as a potential target for developing control strategies for A. niger infection and OTA biosynthesis.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycotoxin Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12550-022-00463-1","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/7/26 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}

引用次数: 3

Abstract

The global regulator LaeA and its orthologs govern the morphogenetic development and secondary metabolism of several filamentous ascomycetes. In Aspergillus niger, it has been shown that an LaeA ortholog (AnLaeA) regulates the production of citric acid and secondary metabolites. In this work, we constructed AnlaeA disruption and overexpression strains to investigate the roles of AnLaeA in morphological development and ochratoxin A (OTA) biosynthesis in A. niger. Phenotypic observation, chemical analysis, and gene expression analysis indicated that AnLaeA acts as a negative regulator of conidial morphogenesis and positively regulates gene expression of the OTA cluster in A. niger grown in CYA medium. However, it was observed that the upregulation of gene expression of the OTA cluster does not necessarily increase OTA production. Our results contribute to a better understanding of the AnlaeA regulatory mechanism and suggest the AnlaeA gene as a potential target for developing control strategies for A. niger infection and OTA biosynthesis.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

LaeA调控黑曲霉的形态发育和赭曲霉毒素A的合成。

全球调节剂LaeA及其同源物控制着几种丝状子囊菌的形态发生发育和次级代谢。在黑曲霉中，已经证明LaeA同源物(AnLaeA)调节柠檬酸和次级代谢物的产生。本研究通过构建AnlaeA裂解菌株和过表达菌株，探讨AnlaeA在黑曲霉霉形态发育和赭曲霉毒素A (OTA)合成中的作用。表型观察、化学分析和基因表达分析表明，AnLaeA对CYA培养基中黑曲霉的分生孢子形态发生起负调控作用，而对OTA簇基因表达起正调控作用。然而，我们观察到OTA集群基因表达的上调并不一定会增加OTA的产生。我们的研究结果有助于更好地理解AnlaeA的调控机制，并建议AnlaeA基因作为制定黑曲霉感染和OTA生物合成控制策略的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Mycotoxin Research MYCOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

6.40

自引率

6.70%

发文量

期刊介绍： Mycotoxin Research, the official publication of the Society for Mycotoxin Research, is a peer-reviewed, scientific journal dealing with all aspects related to toxic fungal metabolites. The journal publishes original research articles and reviews in all areas dealing with mycotoxins. As an interdisciplinary platform, Mycotoxin Research welcomes submission of scientific contributions in the following research fields: - Ecology and genetics of mycotoxin formation - Mode of action of mycotoxins, metabolism and toxicology - Agricultural production and mycotoxins - Human and animal health aspects, including exposure studies and risk assessment - Food and feed safety, including occurrence, prevention, regulatory aspects, and control of mycotoxins - Environmental safety and technology-related aspects of mycotoxins - Chemistry, synthesis and analysis.