Isolation of functional ligninolytic Bacillus aryabhattai from paper mill sludge and its lignin degradation potential

Q1 Immunology and Microbiology Biotechnology Reports Pub Date : 2022-09-01 DOI:10.1016/j.btre.2022.e00755

Anjali Singh , Rajesh Kumar , Annapurna Maurya , Pankaj Chowdhary , Abhay Raj

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引用次数: 4

Abstract

Kraft lignin (KL), is the major pollutant in pulp and paper effluent and due to its heterogeneous structure, it is resistant to the depolymerization process. It has drawn much attention from the researcher due to its challenging degradation process. In this study, a KL-degrading bacterium was isolated and screened from paper mill sludge. This bacterium was identified as ligninolytic Bacillus aryabhattai using biochemical and 16SrRNA gene analysis. B. aryabhattai showed maximum activities of lignin peroxidase-LiP (0.74 IU mL⁻¹) and manganese peroxidase-MnP (9.2 IU mL⁻¹) on the 4th day, and 5th day, respectively. A total 84% of KL (500 mg L⁻¹) reduction was observed after 14 days. The KL bio-degradation was confirmed based on changes in chemical stracture of KL and new metabolites identification using FTIR and GC–MS, respectively. The study concluded that B. aryabhattai maybe becomes a potential biological agent in KL biodegradation and treatment of other lignin-containing industrial effluents.

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从造纸厂污泥中分离功能性木质素降解芽孢杆菌及其木质素降解潜力

硫酸盐木质素(KL)是制浆造纸废水中的主要污染物，由于其非均相结构，对解聚过程具有抗性。由于其具有挑战性的降解过程，引起了研究人员的广泛关注。本研究从造纸厂污泥中分离并筛选了一种kl降解菌。通过生化和16SrRNA基因分析，鉴定该菌为木质素降解芽孢杆菌。木质素过氧化物酶- lip (0.74 IU mL−1)和锰过氧化物酶- mnp (9.2 IU mL−1)活性分别在第4天和第5天达到最大值。14天后，KL (500 mg L−1)减少了84%。利用FTIR和GC-MS分别对KL的化学结构变化和新的代谢物进行鉴定，证实了KL的生物降解。研究结果表明，在KL生物降解及其他含木质素工业废水处理中，木质素菌可能成为一种潜在的生物制剂。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.