Growth hormone inhibits adipogenic differentiation and induces browning in bovine subcutaneous adipocytes

IF 1.6 4区 医学 Q4 CELL BIOLOGY Growth Hormone & Igf Research Pub Date : 2022-10-01 DOI:10.1016/j.ghir.2022.101498
Tingting Li, Hui Bai, Haoyuan Fang, Liang Yang, Peishi Yan
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引用次数: 0

Abstract

Objective

It is well established that growth hormone (GH) has the ability to stimulate lipolysis. The effects of GH on adipocyte differentiation and browning have not been clearly described. Therefore, the present study aimed to elucidate the role of GH in the differentiation and browning of bovine subcutaneous adipocytes as well as its underlying molecular mechanisms.

Methods

We first treated bovine subcutaneous preadipocytes with different concentrations (0, 10, 100, and 500 ng/mL) of GH for 8 days and measured lipid accumulation and gene expression. Afterward, we treated preadipocytes and mature adipocytes with 500 ng/mL GH and determined differentiation and browning-related indicators. Finally, we investigated the expression of STAT5B in both preadipocytes and mature adipocytes after GH treatment.

Results

We demonstrated that GH inhibited lipid accumulation and decreased the expression levels of adipogenic key genes (SCD1, SREBP1, PPARγ, and CEBPα) during adipocyte differentiation. Moreover, we observed that the inhibitory effect of GH on the early stage of adipocyte differentiation (0–2 days) was stronger than that on the later stage of adipocyte differentiation (2–8 days). We also found that GH promoted the expression levels of browning-related genes such as uncoupling protein 1 (UCP1) in mature adipocytes. Concurrently, GH promoted mitochondrial biogenesis and increased the expression levels of mitochondrial biogenesis-related genes. In addition, GH promoted phosphorylation of signal transducers and activator of transcription 5 b (STAT5B) and contributed to translocation of STAT5B to nucleus. After blocking the expression of STAT5B protein, GH weakened the inhibition of adipogenic key genes and reduced the promotion of browning-related genes in bovine subcutaneous adipocytes.

Conclusions

GH can inhibit adipocyte differentiation and promote adipocyte browning by regulating STAT5B in bovine subcutaneous adipocytes.

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生长激素抑制牛皮下脂肪细胞的成脂分化并诱导褐变
目的:生长激素(GH)具有促进脂肪分解的作用。生长激素对脂肪细胞分化和褐变的影响尚未得到明确的描述。因此,本研究旨在阐明生长激素在牛皮下脂肪细胞分化和褐变中的作用及其潜在的分子机制。方法首先用不同浓度的生长激素(0、10、100和500 ng/mL)处理牛皮下前脂肪细胞8天,检测脂肪积累和基因表达。随后,我们用500 ng/mL GH处理前脂肪细胞和成熟脂肪细胞,并测定分化和褐变相关指标。最后,我们研究了GH处理后STAT5B在前脂肪细胞和成熟脂肪细胞中的表达。结果我们发现生长激素抑制脂肪积累,降低脂肪生成关键基因(SCD1、SREBP1、PPARγ和CEBPα)在脂肪细胞分化过程中的表达水平。此外,我们观察到GH对脂肪细胞分化早期(0-2天)的抑制作用强于对脂肪细胞分化后期(2-8天)的抑制作用。我们还发现,生长激素可以促进成熟脂肪细胞中褐化相关基因如解偶联蛋白1 (UCP1)的表达水平。同时,生长激素促进线粒体生物发生,提高线粒体生物发生相关基因的表达水平。此外,GH促进了信号转导和转录激活因子5b (STAT5B)的磷酸化,并促进了STAT5B向细胞核的易位。在阻断STAT5B蛋白表达后,GH减弱了牛皮下脂肪细胞中脂肪生成关键基因的抑制作用,降低了褐变相关基因的促进作用。结论生长激素通过调节牛皮下脂肪细胞STAT5B,抑制脂肪细胞分化,促进脂肪褐变。
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来源期刊
Growth Hormone & Igf Research
Growth Hormone & Igf Research 医学-内分泌学与代谢
CiteScore
3.30
自引率
0.00%
发文量
38
审稿时长
57 days
期刊介绍: Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.
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