Alessandro Usai , Jon K. Pittman , Constantinos Theodoropoulos
{"title":"A multiscale modelling approach for Haematococcus pluvialis cultivation under different environmental conditions","authors":"Alessandro Usai , Jon K. Pittman , Constantinos Theodoropoulos","doi":"10.1016/j.btre.2022.e00771","DOIUrl":null,"url":null,"abstract":"<div><p><em>Haematococcus pluvialis</em> can produce significant amounts of industrially important compounds belonging to lipids and starch classes, including various specific pigments such as β-carotene, lutein and astaxanthin, as well as lipids, carbohydrates and proteins. Their production can vary depending on environmental stress conditions like nutrient starvation. However, stress conditions lead also to undesired phenomena such as cell lysis, which is likely to be related to products loss. The microorganism develops towards smaller single cell volumes during the growth process, and eventually, more likely towards lysis when fission (i.e. cell division) slows down. The lysis process takes place simultaneously with nutrient depletion, so both growth and lysis are linked to the change of environmental conditions. In this work, we develop a novel multiscale segregated-structured model based on Population Balance Equations (PBEs) to describe the photoautotrophic growth of <em>H.pluvialis</em>, in particular cell growth, and lysis, making possible the description of the relationship between cell volume/transition, cell loss, and metabolic product availability. Cell volume is the internal coordinate of the population balance model, and its link with intrinsic concentrations is also presented. The model parameters are fitted against experimental data, extensive sensitivity analysis is performed and the model predictive capabilities are tested in terms of cell density distributions, as well as 0th and 1st order moments.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636539/pdf/","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2215017X22000698","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
引用次数: 1
Abstract
Haematococcus pluvialis can produce significant amounts of industrially important compounds belonging to lipids and starch classes, including various specific pigments such as β-carotene, lutein and astaxanthin, as well as lipids, carbohydrates and proteins. Their production can vary depending on environmental stress conditions like nutrient starvation. However, stress conditions lead also to undesired phenomena such as cell lysis, which is likely to be related to products loss. The microorganism develops towards smaller single cell volumes during the growth process, and eventually, more likely towards lysis when fission (i.e. cell division) slows down. The lysis process takes place simultaneously with nutrient depletion, so both growth and lysis are linked to the change of environmental conditions. In this work, we develop a novel multiscale segregated-structured model based on Population Balance Equations (PBEs) to describe the photoautotrophic growth of H.pluvialis, in particular cell growth, and lysis, making possible the description of the relationship between cell volume/transition, cell loss, and metabolic product availability. Cell volume is the internal coordinate of the population balance model, and its link with intrinsic concentrations is also presented. The model parameters are fitted against experimental data, extensive sensitivity analysis is performed and the model predictive capabilities are tested in terms of cell density distributions, as well as 0th and 1st order moments.
Biotechnology ReportsImmunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
15.80
自引率
0.00%
发文量
79
审稿时长
55 days
期刊介绍:
Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.