Oral magnesium sulphate administration in rats with minimal hepatic encephalopathy: NMR-based metabolic characterization of the brain

IF 1.5 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Magnesium research Pub Date : 2022-04-01 DOI:10.1684/mrh.2022.0500

Xue-Fei Liu, Jin-Jin Lu, Ying Li, Xiu-Ying Yang, Jin-Wei Qiang

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Abstract

Objective: To investigate the metabolic changes in rats with minimal hepatic encephalopathy (MHE) treated with oral magnesium sulphate administration.

Materials and methods: A total of 30 Sprague-Dawley rats were divided into a control group and MHE group (further divided into an MHE group and an MHE-Mg group treated with oral administration of 124 mg/kg/day magnesium sulphate). Morris water maze (MWM), Y maze and narrow beam walking (NBW) were used to evaluate cognitive and motor functions. Brain manganese and magnesium content were measured. The metabolic changes in rats with MHE were investigated using hydrogen-nuclear magnetic resonance. Metabolomic signatures were identified with enrichment and pathway analysis.

Results: A significantly decreased number of entries into the MWM within the range of interest, longer latency and total time during NBW, and higher brain manganese content were found in rats with MHE. After magnesium sulphate treatment, the rats with MHE had better behavioural performance and lower brain manganese content. The 25 and 26 metabolomic signatures were identified in the cortex and striatum of rats with MHE. The pathway analysis revealed alanine, aspartate and glutamate metabolism as the major abnormal metabolic pathways associated with these metabolomic signatures.

Conclusion: Alanine, aspartate and glutamate metabolism are major abnormal metabolic pathways in rats with MHE, which could be restored by magnesium sulphate treatment.

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轻度肝性脑病大鼠口服硫酸镁:基于核磁共振的脑代谢特征

目的:探讨口服硫酸镁对轻度肝性脑病(MHE)大鼠代谢的影响。材料与方法:将30只sd大鼠分为对照组和MHE组(再分为MHE组和MHE- mg组，均口服硫酸镁124 mg/kg/d)。Morris水迷宫(MWM)、Y型迷宫(Y型迷宫)和窄梁步行(NBW)对大鼠的认知和运动功能进行评价。测定脑锰、镁含量。采用氢核磁共振技术研究MHE大鼠的代谢变化。代谢组学特征通过富集和途径分析确定。结果:MHE大鼠进入感兴趣范围内MWM的次数明显减少，NBW期间的潜伏期和总时间更长，脑锰含量较高。硫酸镁处理后，MHE大鼠行为表现较好，脑锰含量较低。在MHE大鼠皮层和纹状体中鉴定了25和26个代谢组学特征。途径分析显示丙氨酸、天冬氨酸和谷氨酸代谢是与这些代谢组学特征相关的主要异常代谢途径。结论:丙氨酸、天冬氨酸和谷氨酸代谢是MHE大鼠的主要代谢异常途径，硫酸镁可使其恢复正常。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Magnesium research 医学-内分泌学与代谢

CiteScore

3.50

自引率

9.40%

发文量

审稿时长

>12 weeks

期刊介绍： Magnesium Research, the official journal of the international Society for the Development of Research on Magnesium (SDRM), has been the benchmark journal on the use of magnesium in biomedicine for more than 30 years. This quarterly publication provides regular updates on multinational and multidisciplinary research into magnesium, bringing together original experimental and clinical articles, correspondence, Letters to the Editor, comments on latest news, general features, summaries of relevant articles from other journals, and reports and statements from national and international conferences and symposiums. Indexed in the leading medical databases, Magnesium Research is an essential journal for specialists and general practitioners, for basic and clinical researchers, for practising doctors and academics.