Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI:10.1267/ahc.22-00062

Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu

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Abstract

The purpose of this study was to determine the role of oxidized diacylglycerol (DAG) and the molecular mechanism underlying ischemia-reperfusion (I/R) injury in rat skin flaps. The protective effect of ebselen on the viability of rat skin flaps with I/R injury was investigated. Flaps were designed and raised in the left inguinal region. Then, a microvascular clamp was applied to the vascular pedicle and reperfused after 6 hr. After 7 days of I/R (I/R group), the skin flap survival area ratio was significantly reduced compared to the normal skin. The administration of ebselen significantly improved the ratio compared to the I/R group. The flap survival area ratio of the I/R + ebselen group was significantly improved compared to the I/R + vehicle group. In the I/R + ebselen group, the oxidized DAG content and intensity of phosphorylated PKCα and PKCδ were significantly lower compared to the I/R + vehicle group. Furthermore, the inflammatory response was suppressed in the I/R + ebselen group compared to the I/R + vehicle group. These results indicate that ebselen is useful as a preventive and therapeutic agent for skin flap necrosis caused by I/R, because of reduction and elimination of oxidized DAG.

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艾布selen对大鼠上腹部皮瓣缺血再灌注损伤的保护作用。

本研究旨在探讨氧化二酰基甘油(DAG)在大鼠皮瓣缺血再灌注(I/R)损伤中的作用及其分子机制。研究了依布selen对I/R损伤大鼠皮瓣存活的保护作用。在左侧腹股沟区设计并抬高皮瓣。然后用微血管钳夹住血管蒂，6小时后再灌注。I/R组在I/R 7 d后，皮瓣存活面积比明显低于正常皮肤。与I/R组相比，艾布selen组显著提高了该比率。与I/R + vehicle组相比，I/R + ebselen组皮瓣存活面积比显著提高。I/R + ebselen组氧化DAG含量和磷酸化PKCα和PKCδ的强度显著低于I/R +载体组。此外，与I/R + vehicle组相比，I/R + ebselen组的炎症反应受到抑制。这些结果表明，依布selen可减少和消除氧化DAG，可作为I/R所致皮瓣坏死的预防和治疗药物。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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