Blood-group-substance activity of bovine submaxillary mucin I. J activity of bovine submaxillary mucin and properties of bovine submaxillary mucin glycopeptides

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-07-07 DOI:10.1016/0926-6526(64)90037-0

Martin I. Horowitz, Yohichi Hashimoto, Ward Pigman

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引用次数: 3

Abstract

1.
1. The presence of blood-group-substance activity in bovine saliva prompted investigation of the immunologic behavior of bovine submaxillary mucin and of glycopeptides obtained by proteolytic digestion of the mucin.
2.
2. Purified preparations of bovine submaxillary mucin inhibited hemolysis in the bovine J-anti-J system. Removal of sialic acid from the mucin by mild acid hydrolysis increased the potency of the preparation. Periodate oxidation of sialic acid free-bovine submaxillary mucin destroyed the blood-group J activity.
3.
3. Bovine submaxillary mucin exhibited a high degree of specificity. it did not inhibit other bovine blood group systems, and it was inactive in human B, M, N and I, i systems; bovine submaxillary mucin had moderate inhibition in the A and O (H) systems.
4.
4. Bovine submaxillary mucin was digested by pronase and papain (EC 3.4.4.10), and the glycopeptides were fractionated through Sephadex G-50. Glycopeptide fractions which contained the greatest amounts of hexosamine, galactose, and fucose relative to sialic acid were the best glycopeptide inhibitors of J hemolysis.
5.
5. Bovine submaxillary mucin cross-reacted with antiserum to Type XIV pneumococcal polysaccharide only after removal of sialic acid from the mucin. Possibly some d-galactosyl residues were exposed by the removal of sialic acid from bovine submaxillary mucin.

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牛颌下粘蛋白的血群物质活性ⅰ。牛颌下粘蛋白的活性和牛颌下粘蛋白糖肽的性质

1.1. 牛唾液中血群物质活性的存在促使了对牛颌下黏液和通过蛋白水解消化黏液获得的糖肽的免疫行为的研究。牛颌下粘蛋白纯化制剂抑制牛j -抗j系统溶血。通过温和的酸水解从粘蛋白中去除唾液酸增加了制备的效力。唾液酸游离牛颌下粘蛋白的高碘酸氧化破坏了血型J的活性。牛颌下粘蛋白表现出高度的特异性。对其它牛血型系统无抑制作用，对人B、M、N和I、I系统无抑制作用;牛颌下粘蛋白对A和O (H)系统有中等抑制作用。用蛋白酶和木瓜蛋白酶(EC 3.4.4.10)消化牛颌下粘蛋白，用Sephadex G-50分离糖肽。与唾液酸相比，糖肽组分中己糖胺、半乳糖和焦糖的含量最高，是J型溶血的最佳糖肽抑制剂。牛颌下粘蛋白只有在去除粘蛋白中的唾液酸后才能与抗血清发生对XIV型肺炎球菌多糖的交叉反应。从牛颌下黏液中去除唾液酸可能暴露了一些d-半乳糖残基。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides

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