Development of a probe DNA which accompanies color change on hybridization.

Abstract

Naphthyl Red moiety, conjugated to DNA, shows distinct chromism by hybridization with its complementary DNA. Single-stranded DNA involving Naphthyl Red moiety exhibits orange color and has lambda max at 466 nm at pH 7.0. Absorption maximum shifts towards 545 nm by the presence of its complementary DNA, and accordingly color of the solution changes from orange to magenta.