Stretching of single DNA molecules by LB technique for restriction site mapping.

Kuniharu Ijiro, Yasutaka Matsuo, Masatsugu Shimomura
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引用次数: 3

Abstract

Stretched single DNA molecules were prepared on substrates by Langmuir-Blodgett (LB) method for the restriction site mapping. Stretched single Lambda DNA and EcoRI bound to the DNA were observed by scanning near-field optical microscope (SNOM). As a result, the specific binding sites of EcoRI on the Lambda DNA molecules were determined.

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用LB技术拉伸单个DNA分子进行限制性位点定位。
采用Langmuir-Blodgett (LB)法在底物上制备拉伸的DNA单分子,用于限制性位点定位。通过扫描近场光学显微镜(SNOM)观察拉伸后的单个Lambda DNA和与DNA结合的EcoRI。结果确定了EcoRI在Lambda DNA分子上的特异性结合位点。
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