New mode of HPLC separation for multi-charged polymers, peptides, and proteins

Abstract

This article explores a new mode of chromatographic separation named BIST™ (Bridge Ion Separation Technique) that is designed specifically for the retention and separation of charged molecules. The proposed mechanism is supported by multiple examples. The separation technique is especially useful for analysis of low molecular weight charged polymers, the class of molecules where ion-exchange, ion-exclusion and size exclusion modes of separation do not work effectively. Among low molecular weight polymers, many compounds are of high importance for biological, medicinal, agricultural, and consumer applications. Some examples include heparin, poly amino acids, insulin, polyethyleneimine, as well as other peptides. The wide variety of applications of many charged polymers necessitates a high-quality analytical technique for testing and research of these compounds.