Rspo1 and Rspo3 are required for sensory lineage neural crest formation in mouse embryos

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY Developmental Dynamics Pub Date : 2023-09-28 DOI:10.1002/dvdy.659
Takuma Shinozuka, Motoko Aoki, Yudai Hatakeyama, Noriaki Sasai, Hitoshi Okamoto, Shinji Takada
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Abstract

Background

R-spondins (Rspos) are secreted proteins that modulate Wnt/β-catenin signaling. At the early stages of spinal cord development, Wnts (Wnt1, Wnt3a) and Rspos (Rspo1, Rspo3) are co-expressed in the roof plate, suggesting that Rspos are involved in development of dorsal spinal cord and neural crest cells in cooperation with Wnt ligands.

Results

Here, we found that Rspo1 and Rspo3, as well as Wnt1 and Wnt3a, maintained roof-plate-specific expression until late embryonic stages. Rspo1- and Rspo3-double-knock-out (dKO) embryos partially exhibited the phenotype of Wnt1 and Wnt3a dKO embryos. While the number of Ngn2-positive sensory lineage neural crest cells is reduced in Rspo-dKO embryos, development of dorsal spinal cord, including its size and dorso-ventral patterning in early development, elongation of the roof plate, and proliferation of ependymal cells, proceeded normally. Consistent with these slight defects, Wnt/β-catenin signaling was not obviously changed in developing spinal cord of dKO embryos.

Conclusions

Our results show that Rspo1 and Rspo3 are dispensable for most developmental processes involving roof plate-derived Wnt ligands, except for specification of a subtype of neural crest cells. Thus, Rspos may modulate Wnt/β-catenin signaling in a context-dependent manner.

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Rspo1和Rspo3是在小鼠胚胎中形成感觉谱系神经嵴所必需的。
背景:R-反应蛋白是调节Wnt/β-catenin信号传导的分泌蛋白。在脊髓发育的早期阶段,Wnts(Wnt1,Wnt3a)和Rspos(Rspo1,Rspo3)在顶板中共表达,这表明Rspos与Wnt配体合作参与脊髓背侧和神经嵴细胞的发育。结果:在这里,我们发现Rspo1和Rspo3,以及Wnt1和Wnt3a,保持屋面板特异性表达直到胚胎晚期。Rspo1-和Rspo3-双敲除(dKO)胚胎部分表现出Wnt1和Wnt3a-dKO胚胎的表型。虽然Rspo-dKO胚胎中Ngn2阳性感觉谱系神经嵴细胞的数量减少,但脊髓背侧的发育,包括其大小和早期发育中的背腹侧模式、顶板的伸长和室管膜细胞的增殖,都正常进行。与这些轻微缺陷一致的是,在dKO胚胎的脊髓发育过程中,Wnt/β-catenin信号传导没有明显改变。结论:我们的研究结果表明,Rspo1和Rspo3在涉及顶板衍生的Wnt配体的大多数发育过程中是可有可无的,除了神经嵴细胞的一种亚型的指定。因此,Rsps可能以上下文依赖的方式调节Wnt/β-catenin信号传导。
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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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