Combined antimicrobial effect of phage-derived endolysin and depolymerase against biofilm-forming Salmonella Typhimurium.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2023-08-01 Epub Date: 2023-10-09 DOI:10.1080/08927014.2023.2265817

Junhwan Kim, Jun Wang, Juhee Ahn

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Abstract

This study was designed to evaluate the antimicrobial activity of phage-derived endolysin (LysPB32) and depolymerase (DpolP22) against planktonic and biofilm cells of Salmonella Typhimurium (ST^KCCM). Compared to the control, the numbers of ST^KCCM were reduced by 4.3 and 5.9 log, respectively, at LysPB32 and LysPB32 + DpolP22 in the presence of polymyxin B (PMB) after 48-h incubation at 37 °C. LysPB32 + DpolP22 decreased the relative fitness (0.8) and the cross-resistance of ST^KCCM to chloramphenicol (CHL), cephalothin (CEP), ciprofloxacin (CIP), and tetracycline (TET) in the presence of PMB. The MIC_trt/MIC_con ratios of CHL, CEP, CIP, PMB, and TET were between 0.25 and 0.50 for LysPB32 + DpolP22 in the presence of PMB. These results suggest that the application of phage-encoded enzymes with antibiotics can be a promising approach for controlling biofilm formation on medical and food-processing equipment. This is noteworthy in that the application of LysPB32 + DpolP22 could increase antibiotic susceptibility and decrease cross-resistance to other antibiotics.

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噬菌体来源的溶血素和解聚酶对形成生物膜的鼠伤寒沙门氏菌的联合抗菌作用。

本研究旨在评估噬菌体来源的溶血素（LysPB32）和解聚酶（DpolP22）对鼠伤寒沙门氏菌（STKCCM）浮游细胞和生物膜细胞的抗菌活性。与对照组相比，在LysPB32和LysPB32处，STKCCM的数量分别减少了4.3和5.9 log + DpolP22在多粘菌素B（PMB）存在下在37℃孵育48小时后 °C。LysPB32 + 在PMB存在下，DpolP22降低了STKCCM对氯霉素（CHL）、头孢噻吩（CEP）、环丙沙星（CIP）和四环素（TET）的相对适应度（0.8）和交叉耐药性。LysPB32的CHL、CEP、CIP、PMB和TET的MICtrt/MICcon比率在0.25和0.50之间 + DpolP22在PMB存在的情况下。这些结果表明，噬菌体编码的酶与抗生素的应用可能是控制医疗和食品加工设备上生物膜形成的一种很有前途的方法。值得注意的是，LysPB32的应用 + DpolP22可增加抗生素敏感性，降低对其他抗生素的交叉耐药性。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.