Bcl-3 regulates T cell function through energy metabolism.

IF 2.9 4区 医学 Q3 IMMUNOLOGY BMC Immunology Pub Date : 2023-10-04 DOI:10.1186/s12865-023-00570-3
Hui Liu, Lin Zeng, Mengmeng Pan, Liwenhui Huang, Hanying Li, Mengxia Liu, Xinqing Niu, Chenguang Zhang, Hui Wang
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Abstract

Background: Bcl-3 is a member of the IκB protein family and an essential modulator of NF-κB activity. It is well established that Bcl-3 is critical for the normal development, survival and differentiation of adaptive immune cells, especially T cells. However, the regulation of immune cell function by Bcl-3 through metabolic pathways has rarely been studied.

Results: In this study, we explored the role of Bcl-3 in the metabolism and function of T cells via the mTOR pathway. We verified that the proliferation of Bcl-3-deficient Jurkat T cells was inhibited, but their activation was promoted, and Bcl-3 depletion regulated cellular energy metabolism by reducing intracellular ATP and ROS production levels and mitochondrial membrane potential. Bcl-3 also regulates cellular energy metabolism in naive CD4+ T cells. In addition, the knockout of Bcl-3 altered the expression of mTOR, Akt, and Raptor, which are metabolism-related genes, in Jurkat cells.

Conclusions: This finding indicates that Bcl-3 may mediate the energy metabolism of T cells through the mTOR pathway, thereby affecting their function. Overall, we provide novel insights into the regulatory role of Bcl-3 in T-cell energy metabolism for the prevention and treatment of immune diseases.

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Bcl-3通过能量代谢调节T细胞功能。
背景:Bcl-3是IκB蛋白家族的成员,也是NF-κB活性的重要调节剂。Bcl-3对适应性免疫细胞,特别是T细胞的正常发育、存活和分化至关重要。然而,Bcl-3通过代谢途径调节免疫细胞功能的研究很少。结果:在本研究中,我们通过mTOR途径探讨了Bcl-3在T细胞代谢和功能中的作用。我们证实Bcl-3缺陷的Jurkat T细胞的增殖受到抑制,但它们的激活被促进,Bcl-3缺失通过降低细胞内ATP和ROS的产生水平以及线粒体膜电位来调节细胞能量代谢。Bcl-3还调节幼稚CD4+T细胞的细胞能量代谢。此外,Bcl-3的敲除改变了Jurkat细胞中代谢相关基因mTOR、Akt和Raptor的表达。结论:Bcl-3可能通过mTOR途径介导T细胞的能量代谢,从而影响其功能。总之,我们对Bcl-3在T细胞能量代谢中的调节作用提供了新的见解,用于预防和治疗免疫疾病。
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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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