Siderophore-producing Pantoea ferrattrahens sp. nov. isolated from a clinical specimen and Pantoea ferramans sp. nov. isolated from soil at the bottom of a pond

Abstract

Two Gram-negative facultative anaerobes were isolated from a sepsis patient with pancreatic cancer (strain PAGU 2156^T) and soil at the bottom of a pond (strain PAGU 2198^T), respectively. These two strains formed haloes around the colonies on chrome azurol S agar plates, indicating the production of siderophores. Two isolates assigned to the genus Pantoea based on the 16S rRNA gene were differentiated from established species by using polymorphic taxonomies. Phylogenetic analysis using four housekeeping genes (gyrB, rpoB, atpD, and infB) showed that strain PAGU 2156^T is closely related to Pantoea cypripedii LMG 2657^T (89.9%) or Pantoea septica LMG 5345^T (95.7%). Meanwhile, strain PAGU 2198^T formed a single clade with Pantoea rodasii DSM 26611^T (93.6%) and Pantoea rwandensis DSM 105076^T (93.3%). The average nucleotide identity values obtained from the draft genome assembly showed ≤90.2% between strain PAGU 2156^T and closely related species and ≤81.5% between strain PAGU 2198^T and closely related species. Based on various phenotypes, biochemical properties, and whole-cell fatty acid composition compared with related species, it was concluded that each strain should be classified as a new species of the genus Pantoea. In this manuscript, Pantoea ferrattrahens sp. nov. and Pantoea ferramans sp. nov. with strain PAGU 2156^T (=NBRC 115930^T = CCUG 76757^T) and strain PAGU 2198^T (=NBRC 114265^T = CCUG 75151^T) are proposed as each type strain.