Introducing Critical Genes in Response to Photodynamic Therapy: A Network Analysis.

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Journal of lasers in medical sciences Pub Date : 2023-08-10 eCollection Date: 2023-01-01 DOI:10.34172/jlms.2023.25
Vahid Mansouri, Babak Arjmand, Maryam Hamzeloo-Moghadam, Mostafa Rezaei Tavirani, Zahra Razzaghi, Alireza Ahmadzadeh, Mohammad Rostami Nejad, Reza Mohamoud Robati
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Abstract

Introduction: Photodynamic therapy (PDT) is applied as an efficient method for preventing the progress of cancers. Light and a photosensitive compound which is known as photosensitizer (PS) are the main parts of PDT. In the present study, molecular events after using PDT in the presence of a super lethal dose of a PS were assessed via protein-protein interaction (PPI) analysis. Methods: Data were extracted from Gene Expression Omnibus (GEO). The gene expression profiles of the treated human Sk-Cha1 cells via PDT were compared with the control cells. Expressed change analysis and PPI network analysis were administrated via Cytoscape software v 3.7.2 to find the critical differentially expressed genes (DEGs). Regulatory relationships between the central DEGs were evaluated and the highlighted genes were identified. Results: The significant amounts of gene expression values were grouped and a few DEGs characterized by tremendously expressed values were identified. EGFR, CANX, HSPA5, MYC, JUN, ITGB1, APP, and CDH1 were highlighted as hub-bottleneck DEGs. EGFR, CDH1, and JUN appeared as a set of SEGs, which play a crucial role in response to PDT in the treated Sk-Cha1 cells. Conclusion: In conclusion, regulatory relationships between EGFR, CDH1, and JUN, which have an effect on the regulation of cellular survival, differentiation, and proliferation, were highlighted in the present investigation.

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光动力疗法中关键基因的引入:网络分析。
引言:光动力疗法(PDT)是一种有效的预防癌症进展的方法。光和一种被称为光敏剂(PS)的光敏化合物是PDT的主要组成部分。在本研究中,通过蛋白质-蛋白质相互作用(PPI)分析评估了在超致死剂量PS存在下使用PDT后的分子事件。方法:从基因表达综合数据库(GEO)中提取数据。将经PDT处理的人Sk-Cha1细胞的基因表达谱与对照细胞进行比较。通过Cytoscape软件v3.7.2进行表达变化分析和PPI网络分析,以找到关键差异表达基因(DEG)。评估了中心DEG之间的调节关系,并鉴定了突出显示的基因。结果:对大量的基因表达值进行了分组,并鉴定出了一些以高度表达值为特征的DEG。EGFR、CANX、HSPA5、MYC、JUN、ITGB1、APP和CDH1被强调为枢纽瓶颈DEG。EGFR、CDH1和JUN作为一组SEG出现,在处理的Sk-Cha1细胞中对PDT的反应中起着至关重要的作用。结论:总之,EGFR、CDH1和JUN之间的调节关系在本研究中得到了强调,它们对细胞存活、分化和增殖的调节有影响。
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来源期刊
Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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