Identification of potential biomarkers for SLE through mRNA expression profiling.

Q2 Health Professions Journal of immunoassay & immunochemistry Pub Date : 2024-01-02 Epub Date: 2024-01-22 DOI:10.1080/15321819.2023.2266013

Sara Kamal Rizk, Amal Alhosary, Enas S Zahran, Samah Awad, Marwa Khalil

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引用次数: 0

Abstract

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease that influences numerous body systems. Furin, tristetraprolin (TTP), and NOD, LRR, and pyrin domain-containing protein 3 (NLRP3) contribute in developing autoimmune illnesses.

Aim: Understandthe role of furin, TTP, and NLRP3 mRNA gene expression in SLE pathogenesis and prognosis. Methods: Total 210 individuals were enrolled, divided in two group: cases and control; 105 participants in each group. Real-time quantitative PCR for furin, TTP,and NLRP3 mRNA gene expression were determined for each subject.

Results: SLE patients showed significantly higher serum furin [median 20.10 (0.0-162.88) in comparison with control group [median 1.10 (0.33-8.64)] with significant pvalue (p < 0.001), for NLRP3 expression [median 7.03 (0.0-282.97) compared to control group [median 1.0 (0.44-9.48)] with significant p value (p = 0.006)but lower TTP [median 2.37 (0.0-30.13)] in comparison with control group [median 7.90 (1.0-29.29)] with significant p value (p < 0.001) . Elevated levels of Furin and NLRP3 and low levels of TTP were linked to increased illness activity.

Conclusion: Furin and NLRP increase in SLE and higher with illness activity. TTP is lowerin SLE and negatively correlates with disease activity.

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通过mRNA表达谱鉴定SLE的潜在生物标志物。

背景：系统性红斑狼疮（SLE）是一种影响多种身体系统的自身免疫性疾病。Furin、三四脯氨酸（TTP）、NOD、LRR和含有pyrin结构域的蛋白3（NLRP3）有助于发展自身免疫性疾病。目的：了解弗林蛋白酶、TTP和NLRP3mRNA基因表达在SLE发病机制和预后中的作用。方法：共210例患者，分为两组：病例组和对照组；每组105名参与者。对每个受试者的弗林蛋白酶、TTP和NLRP3mRNA基因表达进行实时定量PCR。结果：SLE患者血清弗林〔中位数20.10（0.0-162.88）〕明显高于对照组〔中位数1.10（0.33-8.64）〕，p值显著（p p值（p = 0.006），但TTP[中位数2.37（0.0-30.13）]低于对照组[中位数7.90（1.0-29.29）] 结论：Furin和NLRP在SLE患者中增高，且随疾病活动性增高而增高。TTP在SLE中较低，并且与疾病活动性呈负相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of immunoassay & immunochemistry 医学-免疫学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.