Celecoxib attenuates neuroinflammation, reactive astrogliosis and promotes neuroprotection in young rats with experimental hydrocephalus

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of chemical neuroanatomy Pub Date : 2023-09-28 DOI:10.1016/j.jchemneu.2023.102344
Maurício Dutra , Stephanya Covas da Silva , Pâmella da Silva Beggiora Marques , Izadora Oliveira Amaral , Stephanie Naomi Funo de Souza , Luiz Antônio Dutra , Marcelo Volpon Santos , Hélio Rubens Machado , Luiza da Silva Lopes
{"title":"Celecoxib attenuates neuroinflammation, reactive astrogliosis and promotes neuroprotection in young rats with experimental hydrocephalus","authors":"Maurício Dutra ,&nbsp;Stephanya Covas da Silva ,&nbsp;Pâmella da Silva Beggiora Marques ,&nbsp;Izadora Oliveira Amaral ,&nbsp;Stephanie Naomi Funo de Souza ,&nbsp;Luiz Antônio Dutra ,&nbsp;Marcelo Volpon Santos ,&nbsp;Hélio Rubens Machado ,&nbsp;Luiza da Silva Lopes","doi":"10.1016/j.jchemneu.2023.102344","DOIUrl":null,"url":null,"abstract":"<div><p><span>Hydrocephalus<span> is a neurological condition with altered cerebrospinal fluid flow<span> (CSF). The treatment is surgical and the most commonly used procedure is ventricle-peritoneal shunt. However, not all patients can undergo immediate surgery or achieve complete lesion reversal. Neuroprotective measures are valuable in such cases. It was evaluated whether the use of </span></span></span>celecoxib<span><span>, a selective inhibitor of COX-2, associated or not with ventricular-subcutaneous derivation, could offer benefits to the brain structures affected by experimental hydrocephalus. Seven-day-old male Wistar Hannover rats induced by intracisternal injection of kaolin 15% were used, divided into five groups with ten animals each: intact control (C), untreated hydrocephalus (H), hydrocephalus treated with celecoxib 20 mg/kg intraperitoneal (HTC), hydrocephalus treated with shunt (HTS) and hydrocephalus treated with shunt and celecoxib 20 mg/kg intraperitoneal (HTCS). Celecoxib was administered for 21 consecutive days, starting the day after hydrocephalus induction and continuing until the end of the experimental period. The surgery was performed seven days after inducing hydrocephalus. Multiple assessment methods were used, such as behavioral tests (water maze and open field), histological analysis (hematoxylin and eosin), </span>immunohistochemistry<span><span> (caspase-3, COX-2, and GFAP), and ELISA<span> analysis of GFAP. The results of the behavioral and memory tests indicated that celecoxib improves the neurobehavioral response. The improvement can be attributed to the reduced neuroinflammation (p &lt; 0.05), and </span></span>astrogliosis (p &lt; 0.05) in different brain regions. In conclusion, the results suggest that celecoxib holds great potential as an adjuvant neuroprotective drug for the treatment of experimental hydrocephalus.</span></span></p></div>","PeriodicalId":15324,"journal":{"name":"Journal of chemical neuroanatomy","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chemical neuroanatomy","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S089106182300114X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Hydrocephalus is a neurological condition with altered cerebrospinal fluid flow (CSF). The treatment is surgical and the most commonly used procedure is ventricle-peritoneal shunt. However, not all patients can undergo immediate surgery or achieve complete lesion reversal. Neuroprotective measures are valuable in such cases. It was evaluated whether the use of celecoxib, a selective inhibitor of COX-2, associated or not with ventricular-subcutaneous derivation, could offer benefits to the brain structures affected by experimental hydrocephalus. Seven-day-old male Wistar Hannover rats induced by intracisternal injection of kaolin 15% were used, divided into five groups with ten animals each: intact control (C), untreated hydrocephalus (H), hydrocephalus treated with celecoxib 20 mg/kg intraperitoneal (HTC), hydrocephalus treated with shunt (HTS) and hydrocephalus treated with shunt and celecoxib 20 mg/kg intraperitoneal (HTCS). Celecoxib was administered for 21 consecutive days, starting the day after hydrocephalus induction and continuing until the end of the experimental period. The surgery was performed seven days after inducing hydrocephalus. Multiple assessment methods were used, such as behavioral tests (water maze and open field), histological analysis (hematoxylin and eosin), immunohistochemistry (caspase-3, COX-2, and GFAP), and ELISA analysis of GFAP. The results of the behavioral and memory tests indicated that celecoxib improves the neurobehavioral response. The improvement can be attributed to the reduced neuroinflammation (p < 0.05), and astrogliosis (p < 0.05) in different brain regions. In conclusion, the results suggest that celecoxib holds great potential as an adjuvant neuroprotective drug for the treatment of experimental hydrocephalus.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
塞来昔布可减轻实验性脑积水幼鼠的神经炎症、反应性星形胶质细胞增生并促进神经保护。
脑积水是一种伴有脑脊液流量改变的神经系统疾病。治疗是外科手术,最常用的方法是脑室-腹腔分流术。然而,并非所有患者都能立即接受手术或实现完全的病变逆转。在这种情况下,神经保护措施是有价值的。评估塞来昔布(一种选择性COX-2抑制剂)的使用是否与心室皮下衍生有关,是否能对受实验性脑积水影响的大脑结构产生益处。用7天大的雄性Wistar Hannover大鼠,通过脑内注射15%高岭土诱导,分为5组,每组10只动物:完整对照组(C)、未治疗的脑积水组(H)、腹腔内用塞来昔布20mg/kg处理的脑积水(HTC)、用分流器处理的脑出血(HTS)和用分流器和腹腔内用20mg/kg塞来昔布处理的脑水肿(HTCS)。塞来昔布连续给药21天,从脑积水诱导后第二天开始,一直给药到实验期结束。手术是在诱发脑积水7天后进行的。使用多种评估方法,如行为测试(水迷宫和开阔场地)、组织学分析(苏木精和伊红)、免疫组织化学(胱天蛋白酶-3、COX-2和GFAP)和GFAP的ELISA分析。行为和记忆测试结果表明塞来昔布可改善神经行为反应。这种改善可归因于神经炎症的减少(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of chemical neuroanatomy
Journal of chemical neuroanatomy 医学-神经科学
CiteScore
4.50
自引率
3.60%
发文量
87
审稿时长
62 days
期刊介绍: The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.
期刊最新文献
Editorial Board Retraction notice to “Astrocyte response to melatonin treatment in rats under high-carbohydrate high-fat diet” [J. Chem. Neuroanat. 136 (2024) 102389] Retraction notice to “Coenzyme Q10 attenuates neurodegeneration in the cerebellum induced by chronic exposure to tramadol” [J. Chem. Neuroanat. 135 (2024) 102367] Retraction notice to “Maternal diabetes-induced alterations in the expression of brain-derived neurotrophic factor in the developing rat hippocampus” [J. Chem. Neuroanat. 114(2021) 101946] Retraction notice to “Aqueous leaf extract of Phyllanthus amarus protects against oxidative stress and misfiring of dopaminergic neurons in Paraquat-induced Parkinson's disease-like model of adult Wistar rats” [J. Chem. Neuroanat. 135 (2024) 102365]
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1