Comparison of optical density-based growth kinetics for pure culture Campylobacter jejuni, coli and lari grown in blood-free Bolton broth.

IF 1.4 4区 农林科学 Q4 ENVIRONMENTAL SCIENCES Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 Epub Date: 2023-10-12 DOI:10.1080/03601234.2023.2264742
Aaron R Bodie, Michael J Rothrock, Steven C Ricke
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Abstract

Campylobacter growth kinetic parameters can be used to refine the sensitivity and efficiency of microbial growth-based methods. Therefore, the aim of this study was to construct growth curves for C. jejuni, C. coli, and C. lari in pure culture and calculate growth kinetics for each Campylobacter species in the same environmental conditions. Campylobacter jejuni, C. coli and C. lari were grown over 48 h and inoculated into 15 mL Hungate tubes (N = 3 trials per species; 5 biological replicates per trial; 3 species; 1 strain per species). Absorbance measurements were taken in 45 min intervals over 24 h. Optical density readings were plotted versus time to calculate growth kinetic parameters. C. jejuni exhibited the longest lag phase (p < 0.001) at 15 h 20 min ± 30 min, versus C. coli at 11 h 15 min ± 17 min, and C. lari at 9 h 27 min ± 15 min. The exponential phase duration was no longer than 5 h for all species, and doubling times were all less than 1h 30 min. The variation in growth kinetics for the three species of Campylobacter illustrates the importance of determining individual Campylobacter spp. growth responses for optimizing detection based on low bacterial levels. This study provides kinetics and estimates to define enrichment times necessary for low concentration Campylobacter detection.

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在无血Bolton肉汤中生长的纯培养空肠弯曲杆菌、大肠杆菌和拉里菌基于光密度的生长动力学的比较。
弯曲杆菌生长动力学参数可用于改进基于微生物生长的方法的灵敏度和效率。因此,本研究的目的是构建空肠弯曲杆菌、大肠杆菌和拉里弯曲杆菌在纯培养中的生长曲线,并计算每种弯曲杆菌在相同环境条件下的生长动力学。空肠弯曲杆菌、大肠杆菌和拉里梭菌生长超过48 h并接种到15 mL Hungate试管(N = 每种3次试验;每次试验5个生物重复;3种;每种1株)。在45 最小间隔超过24 h.绘制光密度读数与时间的关系图,以计算生长动力学参数。空肠弯曲杆菌表现出最长的滞后期(p 大肠杆菌11 h 15 最小值±17 min和C.lari在9 h 27 最小值±15 min。指数阶段持续时间不超过5 h,倍增次数均小于1h 30 min。三种弯曲杆菌生长动力学的变化说明了确定单个弯曲杆菌的重要性。基于低细菌水平优化检测的生长反应。本研究提供了动力学和估计,以确定低浓度弯曲杆菌检测所需的富集时间。
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来源期刊
CiteScore
4.00
自引率
5.00%
发文量
87
审稿时长
1 months
期刊介绍: 12 issues per year Abstracted/indexed in: Agricola; Analytical Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; BioSciences Information Service of Biological Abstracts (BIOSIS); CAB Abstracts; CAB AGBiotech News and Information; CAB Irrigation & Drainage Abstracts; CAB Soils & Fertilizers Abstracts; Chemical Abstracts Service Plus; CSA Aluminum Industry Abstracts; CSA ANTE: Abstracts in New Technology and Engineering; CSA ASFA 3 Aquatic Pollution and Environmental Quality; CSA ASSIA: Applied Social Sciences Index & Abstracts; CSA Ecology Abstracts; CSA Entomology Abstracts; CSA Environmental Engineering Abstracts; CSA Health & Safety Science Abstracts; CSA Pollution Abstracts; CSA Toxicology Abstracts; CSA Water Resource Abstracts; EBSCOhost Online Research Databases; Elsevier BIOBASE/Current Awareness in Biological Sciences; Elsevier Engineering Information: EMBASE/Excerpta Medica/ Engineering Index/COMPENDEX PLUS; Environment Abstracts; Environmental Knowledge; Food Science and Technology Abstracts; Geo Abstracts; Geobase; Food Science; Index Medicus/ MEDLINE; INIST-Pascal/ CNRS; NIOSHTIC; ISI BIOSIS Previews; Pesticides; Food Contaminants and Agricultural Wastes: Analytical Abstracts; Pollution Abstracts; PubSCIENCE; Reference Update; Research Alert; Science Citation Index Expanded (SCIE); and Water Resources Abstracts.
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