Structure and genetic diversity of Canadian Maritimes wild hops.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Genome Pub Date : 2024-01-01 Epub Date: 2023-09-22 DOI:10.1139/gen-2023-0045
Ashok Somalraju, Braulio Soto-Cerda, Kaushik Ghose, Jason McCallum, Ron Knox, Bourlaye Fofana
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Abstract

Studies on the northeastern American native hops (Humulus lupulus ssp. lupuloides) from the Canadian Maritimes are scarce. This study aimed to evaluate the genetic structure and diversity among 25 wild-collected hops from three Canadian Maritime provinces using microsatellite (simple sequence repeat (SSR)) markers. Based on 43 SSR markers, four distinct subgroups were found, with a low molecular variance (19%) between subgroups and a high variance (81%) within subgroups. The Nei's unbiased genetic distance between clusters ranged from 0.01 to 0.08, the genetic distance between clusters 2 and 3 being the farthest and that between clusters 1 and 2 the closest. Cluster 2 captured the highest overall diversity. A total of 18 SSR markers clearly discriminated hop clones by detecting putative subspecies-specific haplotypes, differentiating clones of native-wild H. lupulus ssp. lupuloides from the naturalized old and modern hop cultivars. Seven of the 18 SSR markers also differentiated two clones from the same site from one another. The study is the first, using molecular markers, to identify SSR markers with potential for intellectual property protection in Canadian Maritimes hops. The SSR markers herein used can be prime tools for hop breeders and growers in the region.

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加拿大Maritimes野生啤酒花的结构和遗传多样性。
对来自加拿大滨海的美国东北部本地啤酒花(Humulus lupulus ssp.lupuloides)的研究很少。本研究旨在利用微卫星(SSR)标记对来自加拿大三个滨海省的25个野生啤酒花的遗传结构和多样性进行评估。基于43个SSR标记,发现了四个不同的亚组,亚组之间的低分子方差(19%)和亚组内部的高方差(81%)。聚类间的Nei无偏遗传距离为0.01~0.08;聚类2与聚类3的遗传距离最远,聚类1与聚类2的遗传距离最近。集群2的总体多样性最高。共有18个SSR标记通过检测假定的亚种特异性单倍型,对本地野生羽扇豆的克隆进行了明确的鉴别。来自归化的古老和现代啤酒花品种的羽扇豆。18个SSR标记中的7个也使来自同一位点的两个克隆相互分化。这项研究首次使用分子标记在加拿大Maritimes啤酒花中鉴定出具有知识产权保护潜力的SSR标记。本文使用的SSR标记可以作为该地区啤酒花育种家和种植者的主要工具。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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