Adolescent intermittent alcohol exposure produces strain-specific cross-sensitization to nicotine and other behavioral adaptations in adulthood in C57BL/6J and DBA/2J mice

IF 3.3 3区心理学 Q1 BEHAVIORAL SCIENCES Pharmacology Biochemistry and Behavior Pub Date : 2023-10-05 DOI:10.1016/j.pbb.2023.173655

Laurel R. Seemiller, Prescilla Garcia-Trevizo, Carlos Novoa, Lisa R. Goldberg, Samantha Murray, Thomas J. Gould

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Abstract

Adolescent alcohol exposure is associated with lasting behavioral changes in humans and in mice. Prior work from our laboratory and others have demonstrated that C57BL/6J and DBA/2J mice differ in sensitivity to some effects of acute alcohol exposure during adolescence and adulthood. However, it is unknown if these strains differ in cognitive, anxiety-related, and addiction-related long-term consequences of adolescent intermittent alcohol exposure. This study examined the impact of a previously validated adolescent alcohol exposure paradigm (2–3 g/kg, i.p., every other day PND 30–44) in C57BL/6J and DBA/2J male and female mice on adult fear conditioning, anxiety-related behavior (elevated plus maze), and addiction-related phenotypes including nicotine sensitivity (hypothermia and locomotor depression) and alcohol sensitivity (loss of righting reflex; LORR). Both shared and strain-specific long-term consequences of adolescent alcohol exposure were found. Most notably, we found a strain-specific alcohol-induced increase in sensitivity to nicotine's hypothermic effects during adulthood in the DBA/2J strain but not in the C57BL/6J strain. Conversely, both strains demonstrated a robust increased latency to LORR during adulthood after adolescent alcohol exposure. Thus, we observed strain-dependent cross-sensitization to nicotine and strain-independent tolerance to alcohol due to adolescent alcohol exposure. Several strain and sex differences independent of adolescent alcohol treatment were also observed. These include increased sensitivity to nicotine-induced hypothermia in the C57BL/6J strain relative to the DBA/2J strain, in addition to DBA/2J mice showing more anxiety-like behaviors in the elevated plus maze relative to the C57BL/6J strain. Overall, these results suggest that adolescent alcohol exposure results in altered adult sensitivity to nicotine and alcohol with some phenotypes mediated by genetic background.

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青少年间歇性酒精暴露在C57BL/6J和DBA/2J小鼠成年后对尼古丁和其他行为适应产生菌株特异性交叉致敏。

青少年饮酒与人类和小鼠的持久行为变化有关。我们实验室和其他人先前的研究表明，C57BL/6J和DBA/2J小鼠在青春期和成年期对急性酒精暴露的某些影响的敏感性不同。然而，尚不清楚这些菌株在青少年间歇性饮酒的认知、焦虑和成瘾相关的长期后果方面是否存在差异。这项研究考察了先前验证的青少年酒精暴露范式（2-3 g/kg，腹腔注射，每隔一天PND 30-44）在C57BL/6J和DBA/2J雄性和雌性小鼠中对成年恐惧条件、焦虑相关行为（升高加迷宫）和成瘾相关表型的影响，包括尼古丁敏感性（体温过低和运动抑制）和酒精敏感性（翻正反射丧失；LORR）。研究发现，青少年酒精暴露的共同和特定压力的长期后果。最值得注意的是，我们在DBA/2J菌株中发现了菌株特异性酒精诱导的成年期对尼古丁低温作用的敏感性增加，但在C57BL/6J菌株中没有。相反，在青少年酒精暴露后的成年期，这两种菌株对LORR的潜伏期都显著增加。因此，我们观察到，由于青少年酒精暴露，对尼古丁的菌株依赖性交叉致敏和对酒精的菌株非依赖性耐受性。还观察到一些与青少年酒精治疗无关的菌株和性别差异。这包括相对于DBA/2J品系，C57BL/6J品系对尼古丁诱导的低温的敏感性增加，此外，相对于C57BL/2J品株，DBA/2J小鼠在升高的正迷宫中表现出更多的焦虑样行为。总的来说，这些结果表明，青少年酒精暴露导致成年人对尼古丁和酒精的敏感性改变，某些表型由遗传背景介导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacology Biochemistry and Behavior 医学-行为科学

CiteScore

6.40

自引率

2.80%

发文量

122

审稿时长

38 days

期刊介绍： Pharmacology Biochemistry & Behavior publishes original reports in the areas of pharmacology and biochemistry in which the primary emphasis and theoretical context are behavioral. Contributions may involve clinical, preclinical, or basic research. Purely biochemical or toxicology studies will not be published. Papers describing the behavioral effects of novel drugs in models of psychiatric, neurological and cognitive disorders, and central pain must include a positive control unless the paper is on a disease where such a drug is not available yet. Papers focusing on physiological processes (e.g., peripheral pain mechanisms, body temperature regulation, seizure activity) are not accepted as we would like to retain the focus of Pharmacology Biochemistry & Behavior on behavior and its interaction with the biochemistry and neurochemistry of the central nervous system. Papers describing the effects of plant materials are generally not considered, unless the active ingredients are studied, the extraction method is well described, the doses tested are known, and clear and definite experimental evidence on the mechanism of action of the active ingredients is provided.