Delivery of SAV-siRNA via Exosomes from Adipose-Derived Stem Cells for the Treatment of Myocardial Infarction.

IF 4.4 4区医学 Q2 CELL & TISSUE ENGINEERING Tissue engineering and regenerative medicine Pub Date : 2023-12-01 Epub Date: 2023-10-06 DOI:10.1007/s13770-023-00588-z

Weizhe Bai, Tianchuan Zhu, Jiebin Zuo, Yang Li, Xi Huang, Gang Li

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Abstract

Background: Myocardial infarction (MI) leads to cardiomyocyte death, poor cardiac remodeling, and heart failure, making it a major cause of mortality and morbidity. To restore cardiac pumping function, induction of cardiomyocyte regeneration has become a focus of academic interest. The Hippo pathway is known to regulate cardiomyocyte proliferation and heart size, and its inactivation allows adult cardiomyocytes to re-enter the cell cycle.

Methods: In this study, we investigated whether exosomes from adipose-derived stem cells (ADSCs) could effectively transfer siRNA for the Hippo pathway regulator Salvador (SAV) into cardiomyocytes to induce cardiomyocyte regeneration in a mouse model of MI.

Results: Our results showed that exosomes loaded with SAV-siRNA effectively transferred siRNA into cardiomyocytes and induced cardiomyocyte re-entry into the cell cycle, while retaining the previously demonstrated therapeutic efficacy of ADSC-derived exosomes to improve post-infarction cardiac function through anti-fibrotic, pro-angiogenic, and other effects.

Conclusions: Our findings suggest that siRNA delivery via ADSC-derived exosomes may be a promising approach for the treatment of MI.

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从脂肪来源的干细胞通过外泌体递送SAV siRNA用于治疗心肌梗死。

背景：心肌梗死（MI）导致心肌细胞死亡、心脏重塑不良和心力衰竭，是导致死亡和发病的主要原因。为了恢复心脏泵送功能，诱导心肌细胞再生已成为学术界关注的焦点。众所周知，Hippo通路可以调节心肌细胞增殖和心脏大小，其失活使成年心肌细胞重新进入细胞周期。方法：本研究中，我们研究了来自脂肪来源干细胞（ADSCs）的外泌体是否能够有效地将Hippo通路调节因子Salvador（SAV）的siRNA转移到心肌细胞中，以诱导MI小鼠模型中的心肌细胞再生周期，同时保留了先前证明的ADSC衍生的外泌体的治疗功效，通过抗纤维化、促血管生成和其他作用改善梗死后的心脏功能。结论：我们的研究结果表明，通过ADSC衍生的外泌体递送siRNA可能是治疗MI的一种有前途的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue engineering and regenerative medicine CELL & TISSUE ENGINEERING-ENGINEERING, BIOMEDICAL

CiteScore

6.80

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Tissue Engineering and Regenerative Medicine (Tissue Eng Regen Med, TERM), the official journal of the Korean Tissue Engineering and Regenerative Medicine Society, is a publication dedicated to providing research- based solutions to issues related to human diseases. This journal publishes articles that report substantial information and original findings on tissue engineering, medical biomaterials, cells therapy, stem cell biology and regenerative medicine.