Down-regulating CD19 surface markers expression correlates with infectious bursal disease virus replication

IF 1.4 3区农林科学 Q4 IMMUNOLOGY Veterinary immunology and immunopathology Pub Date : 2023-10-01 DOI:10.1016/j.vetimm.2023.110658

Shahla Shahsavandi , Samira Torabi, Mohammad Majid Ebrahimi, Mohammad Bagher Ghadiri

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Abstract

The infectious bursal disease virus (IBDV) causes an acute and highly contagious immunosuppressive response in young chickens by targeting B lymphocytes in immune organs. Changes in regulatory T-cell ratio and apoptosis have been demonstrated during IBDV infection in these cells. The possible change in CD19 expression as the precursor of B cells after IBDV replication was detected in this study. Raji cells were infected with an IBDV isolate at MOIs of 1.0 and 3.0. The viral kinetics were determined using the characteristic virus-induced CPE, cell viability, and infectious titer. Induction of apoptosis and also changes in the CD19 expression within the virus infection were assessed by flow cytometry. The Raji cells were found to be susceptible to IBDV infection by producing marked CPEs dependent on MOI. The infectivity titers were determined in intra- and extracellular samples at the defined hours. The kinetics of early IBDV replication in Raji cells were nearly identical for both MOIs, but a significant difference in the infectivity titer was observed at 48 hpi. The quick apoptotic events were observed to be significantly higher in MOI 3.0, which was correlated with the lower virus titer. A significant CD19 expression change in the IBDV-infected Raji cells was revealed. The results suggested that Raji cells mimic the IBDV replication in lymphoid organs and the virus replication is related to CD19 expression frequencies in the lymphoid cells.

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CD19表面标志物表达下调与传染性法氏囊病病毒复制相关。

传染性法氏囊病病毒（IBDV）通过靶向免疫器官中的B淋巴细胞，在幼鸡中引起急性和高度传染性的免疫抑制反应。在IBDV感染期间，这些细胞中的调节性T细胞比率和细胞凋亡发生了变化。本研究检测了IBDV复制后作为B细胞前体的CD19表达的可能变化。用IBDV分离物以1.0和3.0的MOI感染Raji细胞。使用特征性病毒诱导的CPE、细胞活力和感染滴度来确定病毒动力学。通过流式细胞术评估病毒感染中细胞凋亡的诱导以及CD19表达的变化。Raji细胞通过产生依赖于MOI的标记CPE而对IBDV感染敏感。在规定的时间测定细胞内和细胞外样本的感染性滴度。两种MOI在Raji细胞中早期IBDV复制的动力学几乎相同，但在48 hpi时观察到感染性滴度的显著差异。在MOI3.0中观察到快速凋亡事件显著较高，这与较低的病毒滴度相关。CD19在IBDV感染的Raji细胞中的表达发生了显著变化。结果表明，Raji细胞模拟IBDV在淋巴器官中的复制，病毒复制与CD19在淋巴细胞中的表达频率有关。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.