Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells.

Victoria Martínez-Sánchez, M Visitación Calvo, I Viera, J Girón-Calle, J Fontecha, Antonio Pérez-Gálvez
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Abstract

The milk fat globule membrane (MFGM) provides infants and adults with several health benefits. These are not derived solely from its unique composition, but also from arrangement of lipids in the MFGM that, in the case of newborns, could reach the intestine partially intact. Fluorochromes associated with lipid derivatives were used to prove a fusion process between the MFGM and the cellular membrane of differentiated Caco-2 cells. To explore the mechanism of this interaction, incubations of MFGM with Caco-2 cells were carried out in the presence of fusogenic agents or compounds that block other MFGM interaction pathways with cells. Confocal fluorescence microscopy provided visual evidence of the fusion process. Lastly, determination on the lipid profile of cells after their interaction with MFGM indicated a metabolic rearrangement of lipids leading to accumulation of triacylglycerols.

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乳脂球蛋白膜与肠上皮细胞质膜相互作用的机制。
乳脂肪球膜(MFGM)为婴儿和成人提供了多种健康益处。这些不仅来源于其独特的成分,还来源于MFGM中脂质的排列,对于新生儿来说,这些脂质可以部分完整地到达肠道。与脂质衍生物相关的荧光染料用于证明MFGM和分化的Caco-2细胞的细胞膜之间的融合过程。为了探索这种相互作用的机制,MFGM与Caco-2细胞的孵育是在存在融合剂或阻断其他MFGM与细胞相互作用途径的化合物的情况下进行的。共聚焦荧光显微镜提供了融合过程的视觉证据。最后,对细胞与MFGM相互作用后的脂质图谱的测定表明,脂质的代谢重排导致三酰甘油的积累。
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