Multiscale spatial segregation analysis in digital images of biofilms

IF 5.9 Q1 MICROBIOLOGY Biofilm Pub Date : 2023-09-20 DOI:10.1016/j.bioflm.2023.100157
Iztok Dogsa, Ines Mandic-Mulec
{"title":"Multiscale spatial segregation analysis in digital images of biofilms","authors":"Iztok Dogsa,&nbsp;Ines Mandic-Mulec","doi":"10.1016/j.bioflm.2023.100157","DOIUrl":null,"url":null,"abstract":"<div><p>Quantifying the degree of spatial segregation of two bacterial strains in mixed biofilms is an important topic in microbiology. Spatial segregation is dependent on spatial scale as two strains may appear to be well mixed if observed from a distance, but a closer look can reveal strong separation. Typically, this information is encoded in a digital image that represents the binary system, e.g., a microscopy image of a two species biofilm. To decode spatial segregation information, we have developed quantitative measures for evaluating the degree of the spatial scale-dependent segregation of two bacterial strains in a digital image. The constructed algorithm is based on the new segregation measures and overcomes drawbacks of existing approaches for biofilm segregation analysis. The new approach is implemented in a freely available software and was successfully applied to biofilms of two strains and bacterial suspensions for detection of the different spatial scale-dependent segregation levels.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"6 ","pages":"Article 100157"},"PeriodicalIF":5.9000,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/16/main.PMC10542597.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofilm","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590207523000540","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Quantifying the degree of spatial segregation of two bacterial strains in mixed biofilms is an important topic in microbiology. Spatial segregation is dependent on spatial scale as two strains may appear to be well mixed if observed from a distance, but a closer look can reveal strong separation. Typically, this information is encoded in a digital image that represents the binary system, e.g., a microscopy image of a two species biofilm. To decode spatial segregation information, we have developed quantitative measures for evaluating the degree of the spatial scale-dependent segregation of two bacterial strains in a digital image. The constructed algorithm is based on the new segregation measures and overcomes drawbacks of existing approaches for biofilm segregation analysis. The new approach is implemented in a freely available software and was successfully applied to biofilms of two strains and bacterial suspensions for detection of the different spatial scale-dependent segregation levels.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
生物膜数字图像的多尺度空间分离分析
量化两种菌株在混合生物膜中的空间分离程度是微生物学中的一个重要课题。空间分离取决于空间尺度,因为如果从远处观察,两个菌株可能看起来混合得很好,但仔细观察可以发现强烈的分离。通常,该信息被编码在表示二进制系统的数字图像中,例如,两种生物膜的显微镜图像。为了解码空间分离信息,我们开发了定量测量方法来评估数字图像中两个菌株的空间尺度依赖性分离程度。所构建的算法基于新的分离措施,克服了现有生物膜分离分析方法的缺点。新方法在一个免费提供的软件中实现,并成功应用于两种菌株的生物膜和细菌悬浮液,用于检测不同的空间尺度依赖性分离水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
期刊最新文献
Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus Small cyclic dipeptide produced by Lactobacillus rhamnosus with anti-biofilm properties against Streptococcus mutans biofilm Transcatheter aortic valve implantation (TAVI) prostheses in vitro - biofilm formation and antibiotic effects Effects of nanoparticles on anaerobic, anammox, aerobic, and algal-bacterial granular sludge: A comprehensive review Identification and comparison of protein composition of biofilms in response to EGCG from Enterococcus faecalis and Staphylococcus lugdunensis, which showed opposite patterns in biofilm-forming abilities
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1