Analyzing the pressure resistant, sublethal injury and resuscitable viable but non-culturable state population of Escherichia coli, Staphylococcus aureus, Bacillus amyloliquefaciens and Lactiplantibacillus plantarum under high pressure processing.

Dong Yang, Zhidong Jiang, Qiuyu Meng, Shengkang Wang, Hanxu Pan, Lei Rao, Xiaojun Liao
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引用次数: 1

Abstract

This study aimed to analyze and reduce the pressure resistance (PR), sublethal injury (SLI), and viable but non-culturable (VBNC) populations during HPP. Escherichia coli, Staphylococcus aureus, Bacillus amyloliquefaciens and Lactiplantibacillus plantarum were selected for evaluation of PR, SLI and VBNC cell counts and proportions during HPP. The results revealed that the bactericidal efficiency against these strains gradually improved as the processing pressure increased. However, viable bacteria could still be detected, suggesting that there may involve the presence of resistant population that difficult to be killed or revived from SLI. Further detecting the quantity and proportion of PR, SLI and VBNC bacteria found that these state of cells were present during whole HPP treatment. Additionally, the more resistant a bacterial species was to high pressure, the fewer SLI and more resuscitable VBNC (RVBNC) populations it generated, and vice versa. Therefore, correlation analysis was also employed to make the relationship between log reduction, SLI and RVBNC population ratios clearer. The results demonstrated that the log reduction was highly positive correlation with SLI population ratios, and negative correlation with RVBNC population within our detected species at 500 MPa. Furthermore, CO2 and Nisin were employed to combined with HPP to reduce these survivors. Comparing with 233, 218, 241 and 259 MPa for HPP treatment, it took 37, 89, 135 and 229 MPa for HPP + CO2, and 189, 161, 199 and 292 MPa for HPP + Nisin to the first decimal reduction for E. coli, S.aureus, B. amyloliquefaciens and L. plantarum, respectively. The results indicated that HPP combined with CO2 or Nisin could significantly reduce the quantity of PR, SLI, and RVBNC cells during HPP, and provide better bactericidal effects. In conclusion, we quantified the presence of PR, SLI, and VBNC bacteria after high pressure treatment and investigate the effectiveness of HPP combined with CO2 or Nisin to enhance the inactivation of bacteria and reduce the occurrence of PR, SLI, and RVBNC bacteria.

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分析高压处理下大肠杆菌、金黄色葡萄球菌、解淀粉芽孢杆菌和植物乳杆菌的耐压性、亚致死性损伤和可复苏的存活但不可培养状态种群。
本研究旨在分析和减少HPP期间的耐压(PR)、亚致死损伤(SLI)和存活但不可培养(VBNC)种群。选择大肠杆菌、金黄色葡萄球菌、解淀粉芽孢杆菌和植物乳杆菌来评估HPP期间的PR、SLI和VBNC细胞计数和比例。结果表明,随着处理压力的增加,对这些菌株的杀菌效率逐渐提高。然而,仍然可以检测到有活力的细菌,这表明可能存在难以从SLI中杀死或复活的抗性种群。进一步检测PR、SLI和VBNC细菌的数量和比例发现,这些细胞状态在整个HPP处理过程中都存在。此外,一种细菌对高压的抵抗力越强,它产生的SLI就越少,可复苏的VBNC(RVBNC)种群就越多,反之亦然。因此,还采用了相关分析,以使对数减少、SLI和RVBNC种群比率之间的关系更加清晰。结果表明,在500MPa下,对数减少与SLI种群比例高度正相关,与我们检测到的物种中的RVBNC种群呈负相关。此外,CO2和Nisin被用于与HPP相结合以减少这些幸存者。与HPP处理的233、218、241和259 MPa相比,HPP+CO2处理的大肠杆菌、金黄色葡萄球菌、解淀粉芽孢杆菌和植物乳杆菌分别需要37、89、135和229 MPa,HPP+Nisin处理的需要189、161、199和292 MPa。结果表明,HPP与CO2或Nisin联合使用可以显著减少HPP过程中PR、SLI和RVBNC细胞的数量,并提供更好的杀菌效果。总之,我们量化了高压处理后PR、SLI和VBNC细菌的存在,并研究了HPP与CO2或Nisin联合使用增强细菌灭活和减少PR、SLI和RVBNC细菌发生的有效性。
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