{"title":"CRISPR/Cas9-gene editing approaches in plant breeding.","authors":"Himanshu Saini, Rajneesh Thakur, Rubina Gill, Kalpana Tyagi, Manika Goswami","doi":"10.1080/21645698.2023.2256930","DOIUrl":null,"url":null,"abstract":"<p><p>CRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review provides an in-depth look at the various strategies for synthesizing gRNAs for efficient delivery in plant cells, including chemical synthesis and <i>in vitro</i> transcription. It also covers traditional analytical tools and emerging developments in detection methods to analyze CRISPR/Cas9 mediated mutation in plant breeding. Additionally, the review outlines the various analytical tools which are used to detect and analyze CRISPR/Cas9 mediated mutations, such as next-generation sequencing, restriction enzyme analysis, and southern blotting. Finally, the review discusses emerging detection methods, including digital PCR and qPCR. Hence, CRISPR/Cas9 has great potential for transforming agriculture and opening avenues for new advancements in the system for gene editing in plants.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"14 1","pages":"1-17"},"PeriodicalIF":4.5000,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512805/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/21645698.2023.2256930","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/9/19 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
CRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review provides an in-depth look at the various strategies for synthesizing gRNAs for efficient delivery in plant cells, including chemical synthesis and in vitro transcription. It also covers traditional analytical tools and emerging developments in detection methods to analyze CRISPR/Cas9 mediated mutation in plant breeding. Additionally, the review outlines the various analytical tools which are used to detect and analyze CRISPR/Cas9 mediated mutations, such as next-generation sequencing, restriction enzyme analysis, and southern blotting. Finally, the review discusses emerging detection methods, including digital PCR and qPCR. Hence, CRISPR/Cas9 has great potential for transforming agriculture and opening avenues for new advancements in the system for gene editing in plants.
期刊介绍：
GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers.
GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer.
Topics covered include, but are not limited to:
• Production and analysis of transgenic crops
• Gene insertion studies
• Gene silencing
• Factors affecting gene expression
• Post-translational analysis
• Molecular farming
• Field trial analysis
• Commercialization of modified crops
• Safety and regulatory affairs
BIOLOGICAL SCIENCE AND TECHNOLOGY
• Biofuels
• Data from field trials
• Development of transformation technology
• Elimination of pollutants (Bioremediation)
• Gene silencing mechanisms
• Genome Editing
• Herbicide resistance
• Molecular farming
• Pest resistance
• Plant reproduction (e.g., male sterility, hybrid breeding, apomixis)
• Plants with altered composition
• Tolerance to abiotic stress
• Transgenesis in agriculture
• Biofortification and nutrients improvement
• Genomic, proteomic and bioinformatics methods used for developing GM cops
ECONOMIC, POLITICAL AND SOCIAL ISSUES
• Commercialization
• Consumer attitudes
• International bodies
• National and local government policies
• Public perception, intellectual property, education, (bio)ethical issues
• Regulation, environmental impact and containment
• Socio-economic impact
• Food safety and security
• Risk assessments
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