Natto extract inhibits infection caused by the Aujeszky's disease virus in mice

IF 1.9 4区 医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2023-10-10 DOI:10.1111/1348-0421.13099
Junya Kobayashi, Rongduo Wen, Takanobu Nishikawa, Yuka Nunomura, Takehito Suzuki, Yudai Sejima, Toshiya Gokan, Makio Furukawa, Tomoko Yokota, Nanako Osawa, Yoko Sato, Yutaka Nibu, Tetsuya Mizutani, Mami Oba
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Abstract

Aujeszky's disease virus (ADV), also known as Suid alphaherpesvirus 1, which mainly infects swine, causes life-threatening neurological disorders. This disease is a serious global risk factor for economic losses in the swine industry. The development of new anti-ADV drugs is highly anticipated and required. Natto, a traditional Japanese fermented food made from soybeans, is a well-known health food. In our previous study, we confirmed that natto has the potential to inhibit viral infections by severe acute respiratory syndrome coronavirus 2 and bovine alphaherpesvirus 1 through their putative serine protease(s). In this study, we found that an agent(s) in natto functionally impaired ADV infection in cell culture assays. In addition, ADV treated with natto extract lost viral infectivity in the mice. We conducted an HPLC gel-filtration analysis of natto extract and molecular weight markers and confirmed that Fraction No. 10 had ADV-inactivating ability. Furthermore, the antiviral activity of Fraction No. 10 was inhibited by the serine protease inhibitor 4-(2-Aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF). These results also suggest that Fraction No. 10, adjacent to the 12.5 kDa peak of the marker in natto extract, may inactivate ADV by proteolysis. Our findings provide new avenues of research for the prevention of Aujeszky's disease.

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纳豆提取物可抑制小鼠感染奥耶斯基病病毒。
奥耶斯基病病毒(ADV),也称为Suidα疱疹病毒1型,主要感染猪,会导致危及生命的神经系统疾病。这种疾病是造成养猪业经济损失的严重全球风险因素。抗ADV新药的开发备受期待和要求。纳豆是一种由大豆制成的日本传统发酵食品,是一种著名的健康食品。在我们之前的研究中,我们证实纳豆有可能通过其假定的丝氨酸蛋白酶抑制严重急性呼吸综合征冠状病毒2型和牛α疱疹病毒1型的病毒感染。在这项研究中,我们发现纳豆中的一种制剂在细胞培养试验中功能性地损害了ADV感染。此外,用纳豆提取物处理的ADV在小鼠中失去了病毒感染性。我们对纳豆提取物和分子量标记物进行了HPLC凝胶过滤分析,并确认馏分10具有ADV灭活能力。此外,组分10的抗病毒活性被丝氨酸蛋白酶抑制剂4-(2-氨基乙基)苯磺酰氟盐酸盐(AEBSF)抑制。这些结果还表明,与12.5 纳豆提取物中标记物的kDa峰可能通过蛋白水解失活ADV。我们的发现为预防Aujeszky病提供了新的研究途径。
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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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