Investigation of Antioxidant and Anti-inflammatory Properties of Berberine Nanomicelles: In vitro and In vivo Studies.

Marjan Heidarzadeh, Mehriar Amininasab, Seyed Mahdi Rezayat, Seyyedeh Elaheh Mousavi
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Abstract

Introduction: In the present study, neuroprotective effects of berberine (BBR) and berberine nanomicelle (BBR-NM) against lipopolysaccharides (LPS)-induced stress oxidative were investigated, and compared by evaluating their antioxidant and anti-inflammatory activities in PC12 cells, and rat brains. A fast, green, and simple synthesis method was used to prepare BBR-NMs.

Method: The prepared BBR-NMs were then characterized using dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). In vitro experiments were carried out on the LPS-treated PC12 cell lines to investigate the anti-cytotoxic and antioxidant properties of BBR-NM and BBR. The results showed that BBR-NMs with a diameter of ~100 nm had higher protective effects against ROS production and cytotoxicity induced by LPS in PC12 cells in comparison with free BBR.

Results: Moreover, in vivo experiments indicated that the activity levels of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), increased in the brain of LPS-treated rats administrated with BBR-NM at the optimum dose of 100 mg.kg-1. BBR-NM administration also resulted in decreased concentration of lipid peroxidation (MDA) and pro-inflammatory cytokines, such as Serum interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α).

Conclusion: Overall, BBR-NM demonstrated higher neuroprotective effects than free BBR, making it a promising treatment for improving many diseases caused by oxidative stress and inflammation.

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黄连素纳米胶束的抗氧化和抗炎特性研究:体外和体内研究。
引言:本研究研究研究了黄连素(BBR)和黄连素纳米胶束(BBR-NM)对脂多糖(LPS)诱导的应激氧化的神经保护作用,并通过评价其在PC12细胞和大鼠脑中的抗氧化和抗炎活性进行了比较。采用快速、绿色、简单的合成方法制备了BBR-NMs。方法:利用动态光散射(DLS)、透射电子显微镜(TEM)和扫描电子显微镜(SEM)对制备的BBR-NMs进行了表征。在LPS处理的PC12细胞系上进行了体外实验,以研究BBR-NM和BBR的抗细胞毒性和抗氧化性能。结果表明,与游离BBR相比,直径为~100nm的BBR-NMs对LPS诱导的PC12细胞产生ROS和细胞毒性具有更高的保护作用,在LPS处理的大鼠脑中,以100mg.kg-1的最佳剂量给予BBR-NM会增加。BBR-NM给药还导致脂质过氧化(MDA)和促炎细胞因子(如血清白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α))的浓度降低。结论:总体而言,BBR-NM表现出比游离BBR更高的神经保护作用,使其成为改善由氧化应激和炎症引起的许多疾病的有前景的治疗方法。
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