Changes of insulin receptors in high fat and high glucose diet mice with insulin resistance.

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI:10.1080/21623945.2023.2264444
Chen Lei, Jing Wang, Xin Li, Yuan-Yuan Mao, Jian-Qun Yan
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引用次数: 1

Abstract

This study aimed to observe the expression of insulin-signaling molecules in different organs of mice with insulin resistance (IR). Firstly, mice were fed a high-fat and high-sugar diet (HF group) to establish an IR model, and the controls (NF group) were fed with a normal diet. Next, the weight, fasting blood glucose (FBG), serum insulin and insulin tolerance were detected. Pathological changes of liver tissues were observed by H&E staining. The expressions of INSR, IRS-1 and IRS-2 in the liver, skeletal muscle and ovary were measured by qRT-PCR and western blotting. As a result, compared with the NF group, the HF group mice had increased weight, FBG, insulin and IR index after 6-week of feeding as well as a worse performance in the insulin tolerance test and H&E staining showed fatty liver-like changes after 12-week of feeding, exhibited lower expression of INSR, IRS-1 and IRS-2 in the liver of mice at 6 and 12 weeks. The expression of INSR and IRS-1 in skeletal muscle tissues exhibited the same trend, while those in ovary organs showed the opposite trend. These results suggested that the insulin signaling alters in the liver, skeletal muscle and ovary organs with the progress of IR.

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胰岛素抵抗高脂高糖饮食小鼠胰岛素受体的变化。
本研究旨在观察胰岛素信号分子在胰岛素抵抗(IR)小鼠不同器官中的表达。首先,给小鼠喂食高脂肪高糖饮食(HF组)以建立IR模型,对照组(NF组)喂食正常饮食。接下来,检测体重、空腹血糖(FBG)、血清胰岛素和胰岛素耐受性。H&E染色观察肝组织病理变化。通过qRT-PCR和蛋白质印迹法检测INSR、IRS-1和IRS-2在肝脏、骨骼肌和卵巢中的表达。结果,与NF组相比,HF组小鼠在喂食6周后体重、FBG、胰岛素和IR指数增加,并且在胰岛素耐受测试中表现较差,并且H&E染色显示喂食12周后脂肪肝样变化,在6周和12周时小鼠肝脏中INSR、IRS-1和IRS-2的表达较低。INSR和IRS-1在骨骼肌组织中的表达呈现相同的趋势,而在卵巢器官中的表达则呈现相反的趋势。这些结果表明,胰岛素信号在肝脏、骨骼肌和卵巢器官中随着IR的进展而改变。
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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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