Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-13 DOI:10.1007/s10529-023-03428-1

Zhou Deng, Yinghui Mu, Zhi Chen, Lishi Yan, Xin Ju, Liangzhi Li

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Abstract

Purpose: Erythritol is a valuable compound as sweetener and chemical material however cannot be fermented from the abundant substrate xylose.

Methods: The strain Trichosporonoides oedocephalis ATCC 16958 was employed to produce polyols including xylitol and erythritol by metabolic engineering approaches.

Results: The introduction of a substrate-specific ribose-5-phosphate isomerase endowed T. oedocephalis with xylose-assimilation activity to produce xylitol, and eliminated glycerol production simultaneously. A more value-added product, erythritol was produced by further introducing a homologous xylulose kinase. The carbon flux was redirected from xylitol to erythritol by adding high osmotic pressure. The production of erythritol was improved to 46.5 g/L in flasks by fermentation adjustment, and the process was scaled up in a 5-L fermentor, with a 40 g/L erythritol production after 120 h, and a time-space yield of 0.56 g/L/h.

Conclusion: This study demonstrated the potential of T. oedocephalis in the synthesis of multiple useful products from xylose.

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在红头毛孢子虫ATCC 16958中构建木糖代谢途径，用于生产赤藓糖醇和木糖醇。

目的：赤藓糖醇是一种有价值的甜味剂和化学原料，但不能从丰富的底物木糖中发酵。方法：采用代谢工程方法，以红头毛孢子虫ATCC 16958菌株为原料，生产木糖醇和赤藓糖醇等多元醇。结果：底物特异性核糖-5-磷酸异构酶的引入赋予红头锥虫产生木糖醇的木糖同化活性，同时消除了甘油的产生。通过进一步引入同源木酮糖激酶来生产更具附加值的产品赤藓糖醇。通过添加高渗压将碳通量从木糖醇改性为赤藓糖醇。通过发酵调节，在烧瓶中赤藓糖醇的产量提高到46.5g/L，并在5L发酵罐中扩大了这一过程，120小时后赤藓糖醛产量为40g/L，时空产率为0.56g/L。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.