Sequence-type diversity of invasive Streptococcus pneumoniae isolates in Iran among children under 15 years of age.

IF 1.6 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH GMS Hygiene and Infection Control Pub Date : 2023-09-07 eCollection Date: 2023-01-01 DOI:10.3205/dgkh000445

Sedigheh Rafiei Tabatabaei, Marjan Tariverdi, Abdollah Karimi, Ali Nazari-Alam, Hannan Khodaei, Leila Azimi

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Abstract

Background: Infection with viruses, bacteria, or other pathogens can lead to inflammation of the meninges. Finding the pathogen and identifying the most common type is necessary for each country. Using multi-locus sequence typing (MLST), the aim of this study was to determine the genetic relationship among S. pneumoniae isolated from CSF in children with bacterial meningitis.

Materials and methods: : Fourteen isolates of S. pneumoniae from CSF in children with bacterial meningitis were included in this study. The seven housekeeping genes, primer, and analysis of the sequencing used in MLST were extracted from PubMLST.

Results: The sequencing analysis showed four MLST types in the studied strains. The most frequent type is ST13649 and the least frequent are ST708 and ST285.

Conclusion: Finding the bacterial sequence types (ST) enables comparing the ST in different, especially neighbouring, countries.

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伊朗15岁以下儿童中侵袭性肺炎链球菌分离株的序列类型多样性。

背景：感染病毒、细菌或其他病原体可导致脑膜炎症。每个国家都有必要找到病原体并确定最常见的类型。本研究采用多基因座序列分型法（MLST），旨在确定从儿童细菌性脑膜炎脑脊液中分离的肺炎链球菌之间的遗传关系。材料和方法：本研究从儿童细菌性脑膜炎的脑脊液中分离出14株肺炎链球菌。从PubMLST中提取了MLST中使用的7个管家基因、引物和测序分析。结果：测序分析显示，研究菌株中存在四种MLST类型。最常见的类型是ST13649，最不常见的是ST708和ST285。结论：找到细菌序列类型（ST）可以比较不同国家，尤其是邻国的ST。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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GMS Hygiene and Infection Control PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

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