Inflammatory markers in exhaled breath condensate in nonasthmatic children with food allergy.

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2023-11-09 DOI:10.1088/1752-7163/ad02b5
Ozge Yilmaz, Seda Tunca, Adem Yasar, Merve Ocalan, Fatma Taneli, Hasan Yuksel
{"title":"Inflammatory markers in exhaled breath condensate in nonasthmatic children with food allergy.","authors":"Ozge Yilmaz, Seda Tunca, Adem Yasar, Merve Ocalan, Fatma Taneli, Hasan Yuksel","doi":"10.1088/1752-7163/ad02b5","DOIUrl":null,"url":null,"abstract":"<p><p>Allergy is a systemic inflammation; therefore, although the allergic symptom may be seen in a specific organ system, the effects of this inflammation may be seen in other organs. interleukin (IL) IL4, IL5 and IL13 are the major Th2 cytokines and e-cadherin is an epithelial barrier protein. The objective of this research was to assess indicators of inflammation specific to Th2 responses and proteins related to the protective barrier of the airway's inner lining. These assessments were conducted using exhaled breath condensate (EBC), which provides insights into peripheral airway conditions of children suffering from food allergies. The study had 24 patients with food allergy and 24 control individuals younger than three years of age with no history of food reaction. The diagnosis of food allergy was based on food allergen-specific IgE and skin prick test positivity in our clinic and oral food testing in selected cases. EBC samples were obtained by Ecoscreen (Jaegar, Hoechberg, Germany). IL4, IL5, IL13 and E-cadherin levels were measured in these samples by enzyme linked immunoassay. The group of children with food allergies, consisting mainly of 14 girls, had a median age of 16 months, whereas the control group, which included 11 girls, had a median age of 15 months (<i>p</i>= 0.89). Comparing the two groups, children with food allergies exhibited notably lower levels of IL-13 in the EBC compared to the control group (median values of 59.14 and 76.36, respectively,<i>p</i>= 0.02). Conversely, the concentration of IL-4 in the EBC was significantly higher in children with food allergies (median values of 1.94 and 1.29, respectively,<i>p</i>= 0.003). However, the levels of IL-5 and e-cadherin showed no significant differences between the two groups (with<i>p</i>-values of 0.74 and 0.09, respectively) as shown in table1. High level of IL-4 despite the low level of IL-13 in the EBC of children having food allergy may be indicative of an early inflammatory phase that is not yet in the effector phase. Studies about the evolution of this process later in life are needed to assess the role of airway inflammation in children with food allergy who develop asthma.</p>","PeriodicalId":15306,"journal":{"name":"Journal of breath research","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of breath research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1088/1752-7163/ad02b5","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Allergy is a systemic inflammation; therefore, although the allergic symptom may be seen in a specific organ system, the effects of this inflammation may be seen in other organs. interleukin (IL) IL4, IL5 and IL13 are the major Th2 cytokines and e-cadherin is an epithelial barrier protein. The objective of this research was to assess indicators of inflammation specific to Th2 responses and proteins related to the protective barrier of the airway's inner lining. These assessments were conducted using exhaled breath condensate (EBC), which provides insights into peripheral airway conditions of children suffering from food allergies. The study had 24 patients with food allergy and 24 control individuals younger than three years of age with no history of food reaction. The diagnosis of food allergy was based on food allergen-specific IgE and skin prick test positivity in our clinic and oral food testing in selected cases. EBC samples were obtained by Ecoscreen (Jaegar, Hoechberg, Germany). IL4, IL5, IL13 and E-cadherin levels were measured in these samples by enzyme linked immunoassay. The group of children with food allergies, consisting mainly of 14 girls, had a median age of 16 months, whereas the control group, which included 11 girls, had a median age of 15 months (p= 0.89). Comparing the two groups, children with food allergies exhibited notably lower levels of IL-13 in the EBC compared to the control group (median values of 59.14 and 76.36, respectively,p= 0.02). Conversely, the concentration of IL-4 in the EBC was significantly higher in children with food allergies (median values of 1.94 and 1.29, respectively,p= 0.003). However, the levels of IL-5 and e-cadherin showed no significant differences between the two groups (withp-values of 0.74 and 0.09, respectively) as shown in table1. High level of IL-4 despite the low level of IL-13 in the EBC of children having food allergy may be indicative of an early inflammatory phase that is not yet in the effector phase. Studies about the evolution of this process later in life are needed to assess the role of airway inflammation in children with food allergy who develop asthma.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
食物过敏的非哮喘儿童呼出气体冷凝液中的炎症标志物。
摘要 ;简介:过敏是一种全身性炎症;因此,尽管过敏症状可能出现在特定的器官系统中,但这种炎症的影响可能出现在其他器官中。IL4、IL5和IL13是主要的Th2细胞因子并且e-粘附素是上皮屏障蛋白。本研究的目的是评估Th2反应特异性炎症指标和与气道内衬保护屏障相关的蛋白质。这些评估是使用呼出的冷凝液进行的,它可以深入了解食物过敏儿童的局部气道状况 ;材料和方法:本研究有24名食物过敏患者和24名三岁以下无食物反应史的对照者。食物过敏的诊断是基于我们诊所的食物过敏原特异性IgE和皮肤点刺试验阳性,以及选定病例的口服食物试验。呼气冷凝物(EBC)样品通过Ecoscreen(Jaegar,Hoechberg,德国)获得。通过ELISA测定这些样品中的IL4-5-13和E-钙粘蛋白水平 ;结果:食物过敏儿童组(主要由14名女孩组成)的中位年龄为16个月,而对照组(包括11名女孩)的中位数年龄为15个月(p=0.89),与对照组相比,食物过敏儿童呼出的呼气冷凝液(EBC)中的IL-13水平显著较低(中值分别为59.14和76.36,p=0.02)。相反,食物过敏的儿童呼出的EBC中的IL-4浓度显著较高(中值分别是1.94和1.29,p=0.003)。然而,IL-5和e-钙粘蛋白的水平在两组之间没有显示出显著差异(p值分别为0.74和0.09),如表1所示;结论:尽管食物过敏儿童的EBC中IL-13水平较低,但IL-4水平较高,这可能表明早期炎症阶段尚未进入效应期。需要对这一过程在以后生活中的演变进行研究,以评估气道炎症在食物过敏儿童哮喘中的作用 ;关键词:IL-4,IL-5,IL-13,E-钙粘蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
期刊最新文献
Therapeutic efficacy of a probiotic preparation on idiopathic halitosis: a retrospective observational study. Halitosis in oral lichen planus patients. Effectiveness of a combination of laccase and green coffee extract on oral malodor: A comparative, randomized, controlled, evaluator-blind, parallel-group trial. Validation of a sensor system for the measurement of breath ammonia using selected-ion flow-tube mass spectrometry. Identifying viral infections through analysis of head space volatile organic compounds.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1